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Apheresis for collection of Ebola convalescent plasma in Liberia.

Publication ,  Journal Article
Brown, JF; Rowe, K; Zacharias, P; van Hasselt, J; Dye, JM; Wohl, DA; Fischer, WA; Cunningham, CK; Thielman, NM; Hoover, DL
Published in: J Clin Apher
June 2017

PURPOSE: This report describes initiation of apheresis capability in Liberia, Africa to support a clinical trial of convalescent plasma therapy for Ebola Virus Disease. METHODS: A bloodmobile was outfitted in the United States as a four-bed apheresis unit with capabilities including pathogen reduction, electronic blood establishment computer system, designated areas for donor counseling and laboratory testing, and onboard electrical power generation. After air transport to Liberia, the bloodmobile was positioned at ELWA Hospital, Monrovia, and connected to the hospital's power grid. Liberian staff were trained to conduct donor screening, which included questionnaire and onsite blood typing and transfusion transmitted infection (TTI) testing, and plasma collection and processing. RESULTS: The bloodmobile was operational within 3 weeks after arrival of the advance team. Of 101 donors who passed the pre-screening questionnaire, 32 were deferred. Twenty-eight of ninty-nine tested survivors were deferred for positive transfusion transmitted infection (TTI) tests; twenty-one were positive for hepatitis B, hepatitis C, or human immunodeficiency virus. The majority of donors had type O blood; all but one were Rh positive. Forty-three survivors donated at least once; eighty-nine apheresis attempts resulted in eighty-one successful collections. CONCLUSIONS: Apheresis capability was emergently established in Liberia to support an efficacy trial of Ebola Convalescent Plasma. Extensive cooperation among multinational team members, engineers, logisticians, and blood safety technical personnel at the operational site was required to surmount challenges to execution posed by logistical factors. The high proportion of positive TTI tests supported the use of a pathogen reduction system to enhance product safety. J. Clin. Apheresis 32:175-181, 2017. © 2016 Wiley Periodicals, Inc.

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Published In

J Clin Apher

DOI

EISSN

1098-1101

Publication Date

June 2017

Volume

32

Issue

3

Start / End Page

175 / 181

Location

United States

Related Subject Headings

  • Virus Diseases
  • Survivors
  • Plasma
  • Mass Screening
  • Liberia
  • Humans
  • Hemorrhagic Fever, Ebola
  • Convalescence
  • Cardiovascular System & Hematology
  • Blood Safety
 

Citation

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Brown, J. F., Rowe, K., Zacharias, P., van Hasselt, J., Dye, J. M., Wohl, D. A., … Hoover, D. L. (2017). Apheresis for collection of Ebola convalescent plasma in Liberia. J Clin Apher, 32(3), 175–181. https://doi.org/10.1002/jca.21482
Brown, Jerry F., Kathleen Rowe, Peter Zacharias, James van Hasselt, John M. Dye, David A. Wohl, William A. Fischer, Coleen K. Cunningham, Nathan M. Thielman, and David L. Hoover. “Apheresis for collection of Ebola convalescent plasma in Liberia.J Clin Apher 32, no. 3 (June 2017): 175–81. https://doi.org/10.1002/jca.21482.
Brown JF, Rowe K, Zacharias P, van Hasselt J, Dye JM, Wohl DA, et al. Apheresis for collection of Ebola convalescent plasma in Liberia. J Clin Apher. 2017 Jun;32(3):175–81.
Brown, Jerry F., et al. “Apheresis for collection of Ebola convalescent plasma in Liberia.J Clin Apher, vol. 32, no. 3, June 2017, pp. 175–81. Pubmed, doi:10.1002/jca.21482.
Brown JF, Rowe K, Zacharias P, van Hasselt J, Dye JM, Wohl DA, Fischer WA, Cunningham CK, Thielman NM, Hoover DL. Apheresis for collection of Ebola convalescent plasma in Liberia. J Clin Apher. 2017 Jun;32(3):175–181.
Journal cover image

Published In

J Clin Apher

DOI

EISSN

1098-1101

Publication Date

June 2017

Volume

32

Issue

3

Start / End Page

175 / 181

Location

United States

Related Subject Headings

  • Virus Diseases
  • Survivors
  • Plasma
  • Mass Screening
  • Liberia
  • Humans
  • Hemorrhagic Fever, Ebola
  • Convalescence
  • Cardiovascular System & Hematology
  • Blood Safety