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NanoVelcro Chip for CTC enumeration in prostate cancer patients.

Publication ,  Journal Article
Lu, Y-T; Zhao, L; Shen, Q; Garcia, MA; Wu, D; Hou, S; Song, M; Xu, X; Ouyang, W-H; Ouyang, WW-L; Lichterman, J; Luo, Z; Xuan, X; Huang, J ...
Published in: Methods
December 1, 2013

Circulating tumor cells (CTCs) are one of the most crucial topics in rare cell biology and have become the focus of a significant and emerging area of cancer research. While CTC enumeration is a valid biomarker in prostate cancer, the current FDA-approved CTC technology is unable to detect CTCs in a large portion of late stage prostate cancer patients. Here we introduce the NanoVelcro CTC Chip, a device composed of a patterned silicon nanowire substrate (SiNW) and an overlaid polydimethylsiloxane (PDMS) chaotic mixer. Validated by two institutions participating in the study, the NanoVelcro Chip assay exhibits very consistent efficiency in CTC-capture from patient samples. The utilized protocol can be easily replicated at different facilities. We demonstrate the clinical utility of the NanoVelcro Chip by performing serial enumerations of CTCs in prostate cancer patients after undergoing systemic therapy. Changes in CTC numbers after 4-10 weeks of therapy were compared with their clinical responses. We observed a statistically significant reduction in CTCs counts in the clinical responders. We performed long-term follow up with serial CTC collection and enumeration in one patient observing variations in counts correlating with treatment response. This study demonstrates the consistency of the NanoVelcro Chip assay over time for CTC enumeration and also shows that continuous monitoring of CTC numbers can be employed to follow responses to different treatments and monitor disease progression.

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Published In

Methods

DOI

EISSN

1095-9130

Publication Date

December 1, 2013

Volume

64

Issue

2

Start / End Page

144 / 152

Location

United States

Related Subject Headings

  • Prostatic Neoplasms
  • Prognosis
  • Neoplastic Cells, Circulating
  • Middle Aged
  • Microfluidic Analytical Techniques
  • Male
  • Immunohistochemistry
  • Humans
  • Cell Count
  • Aged, 80 and over
 

Citation

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Lu, Y.-T., Zhao, L., Shen, Q., Garcia, M. A., Wu, D., Hou, S., … Posadas, E. M. (2013). NanoVelcro Chip for CTC enumeration in prostate cancer patients. Methods, 64(2), 144–152. https://doi.org/10.1016/j.ymeth.2013.06.019
Lu, Yi-Tsung, Libo Zhao, Qinglin Shen, Mitch A. Garcia, Dongxia Wu, Shuang Hou, Min Song, et al. “NanoVelcro Chip for CTC enumeration in prostate cancer patients.Methods 64, no. 2 (December 1, 2013): 144–52. https://doi.org/10.1016/j.ymeth.2013.06.019.
Lu Y-T, Zhao L, Shen Q, Garcia MA, Wu D, Hou S, et al. NanoVelcro Chip for CTC enumeration in prostate cancer patients. Methods. 2013 Dec 1;64(2):144–52.
Lu, Yi-Tsung, et al. “NanoVelcro Chip for CTC enumeration in prostate cancer patients.Methods, vol. 64, no. 2, Dec. 2013, pp. 144–52. Pubmed, doi:10.1016/j.ymeth.2013.06.019.
Lu Y-T, Zhao L, Shen Q, Garcia MA, Wu D, Hou S, Song M, Xu X, Ouyang W-H, Ouyang WW-L, Lichterman J, Luo Z, Xuan X, Huang J, Chung LWK, Rettig M, Tseng H-R, Shao C, Posadas EM. NanoVelcro Chip for CTC enumeration in prostate cancer patients. Methods. 2013 Dec 1;64(2):144–152.
Journal cover image

Published In

Methods

DOI

EISSN

1095-9130

Publication Date

December 1, 2013

Volume

64

Issue

2

Start / End Page

144 / 152

Location

United States

Related Subject Headings

  • Prostatic Neoplasms
  • Prognosis
  • Neoplastic Cells, Circulating
  • Middle Aged
  • Microfluidic Analytical Techniques
  • Male
  • Immunohistochemistry
  • Humans
  • Cell Count
  • Aged, 80 and over