Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror.
By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.
Duke Scholars
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Related Subject Headings
- Water
- Sensitivity and Specificity
- Reproducibility of Results
- Photoacoustic Techniques
- Optics
- Microscopy, Confocal
- Micro-Electrical-Mechanical Systems
- Mice
- Lenses
- Image Enhancement
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Water
- Sensitivity and Specificity
- Reproducibility of Results
- Photoacoustic Techniques
- Optics
- Microscopy, Confocal
- Micro-Electrical-Mechanical Systems
- Mice
- Lenses
- Image Enhancement