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Label-free oxygen-metabolic photoacoustic microscopy in vivo.

Publication ,  Journal Article
Yao, J; Maslov, KI; Zhang, Y; Xia, Y; Wang, LV
Published in: Journal of biomedical optics
July 2011

Almost all diseases, especially cancer and diabetes, manifest abnormal oxygen metabolism. Accurately measuring the metabolic rate of oxygen (MRO(2)) can be helpful for fundamental pathophysiological studies, and even early diagnosis and treatment of disease. Current techniques either lack high resolution or rely on exogenous contrast. Here, we propose label-free metabolic photoacoustic microscopy (mPAM) with small vessel resolution to noninvasively quantify MRO(2) in vivo in absolute units. mPAM is the unique modality for simultaneously imaging all five anatomical, chemical, and fluid-dynamic parameters required for such quantification: tissue volume, vessel cross-section, concentration of hemoglobin, oxygen saturation of hemoglobin, and blood flow speed. Hyperthermia, cryotherapy, melanoma, and glioblastoma were longitudinally imaged in vivo. Counterintuitively, increased MRO(2) does not necessarily cause hypoxia or increase oxygen extraction. In fact, early-stage cancer was found to be hyperoxic despite hypermetabolism.

Duke Scholars

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Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

July 2011

Volume

16

Issue

7

Start / End Page

076003

Related Subject Headings

  • Oxygen
  • Optics
  • Neoplasm Transplantation
  • Microvessels
  • Microscopy, Acoustic
  • Mice, Nude
  • Mice
  • Melanoma, Experimental
  • Male
  • Hyperkalemia
 

Citation

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Yao, J., Maslov, K. I., Zhang, Y., Xia, Y., & Wang, L. V. (2011). Label-free oxygen-metabolic photoacoustic microscopy in vivo. Journal of Biomedical Optics, 16(7), 076003. https://doi.org/10.1117/1.3594786
Yao, Junjie, Konstantin I. Maslov, Yu Zhang, Younan Xia, and Lihong V. Wang. “Label-free oxygen-metabolic photoacoustic microscopy in vivo.Journal of Biomedical Optics 16, no. 7 (July 2011): 076003. https://doi.org/10.1117/1.3594786.
Yao J, Maslov KI, Zhang Y, Xia Y, Wang LV. Label-free oxygen-metabolic photoacoustic microscopy in vivo. Journal of biomedical optics. 2011 Jul;16(7):076003.
Yao, Junjie, et al. “Label-free oxygen-metabolic photoacoustic microscopy in vivo.Journal of Biomedical Optics, vol. 16, no. 7, July 2011, p. 076003. Epmc, doi:10.1117/1.3594786.
Yao J, Maslov KI, Zhang Y, Xia Y, Wang LV. Label-free oxygen-metabolic photoacoustic microscopy in vivo. Journal of biomedical optics. 2011 Jul;16(7):076003.

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

July 2011

Volume

16

Issue

7

Start / End Page

076003

Related Subject Headings

  • Oxygen
  • Optics
  • Neoplasm Transplantation
  • Microvessels
  • Microscopy, Acoustic
  • Mice, Nude
  • Mice
  • Melanoma, Experimental
  • Male
  • Hyperkalemia