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Photoacoustic imaging of single circulating melanoma cells in vivo

Publication ,  Conference
Wang, L; Yao, J; Zhang, R; Xu, S; Li, G; Zou, J; Wang, LV
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
March 11, 2015

Melanoma, one of the most common types of skin cancer, has a high mortality rate, mainly due to a high propensity for tumor metastasis. The presence of circulating tumor cells (CTCs) is a potential predictor for metastasis. Label-free imaging of single circulating melanoma cells in vivo provides rich information on tumor progress. Here we present photoacoustic microscopy of single melanoma cells in living animals. We used a fast-scanning optical-resolution photoacoustic microscope to image the microvasculature in mouse ears. The imaging system has sub-cellular spatial resolution and works in reflection mode. A fast-scanning mirror allows the system to acquire fast volumetric images over a large field of view. A 500-kHz pulsed laser was used to image blood and CTCs. Single circulating melanoma cells were imaged in both capillaries and trunk vessels in living animals. These high-resolution images may be used in early detection of CTCs with potentially high sensitivity. In addition, this technique enables in vivo study of tumor cell extravasation from a primary tumor, which addresses an urgent pre-clinical need.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628414134

Publication Date

March 11, 2015

Volume

9323
 

Citation

APA
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Wang, L., Yao, J., Zhang, R., Xu, S., Li, G., Zou, J., & Wang, L. V. (2015). Photoacoustic imaging of single circulating melanoma cells in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9323). https://doi.org/10.1117/12.2076987
Wang, L., J. Yao, R. Zhang, S. Xu, G. Li, J. Zou, and L. V. Wang. “Photoacoustic imaging of single circulating melanoma cells in vivo.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 9323, 2015. https://doi.org/10.1117/12.2076987.
Wang L, Yao J, Zhang R, Xu S, Li G, Zou J, et al. Photoacoustic imaging of single circulating melanoma cells in vivo. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Wang, L., et al. “Photoacoustic imaging of single circulating melanoma cells in vivo.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9323, 2015. Scopus, doi:10.1117/12.2076987.
Wang L, Yao J, Zhang R, Xu S, Li G, Zou J, Wang LV. Photoacoustic imaging of single circulating melanoma cells in vivo. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Journal cover image

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628414134

Publication Date

March 11, 2015

Volume

9323