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Photo-imprint super-resolution photoacoustic microscopy

Publication ,  Conference
Yao, J; Wang, L; Li, C; Zhang, C; Wang, LV
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
March 11, 2015

Combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, we demonstrate a simple method for super-resolution photoacoustic imaging of both fluorescent and non-fluorescent samples. Our method is based on a double-excitation process, where the first excitation pulse partially and inhomogeneously bleaches the molecules in the diffraction-limited excitation volume, thus biasing the signal contributions from a second excitation pulse striking the same region. By scanning the excitation beam, we performed three-dimensional sub-diffraction imaging of varied fluorescent and non-fluorescent species. A lateral resolution of 80 nm and an axial resolution of 370 nm have been demonstrated. This technique has the potential to enable label-free super-resolution imaging, and can be transferred to other optical imaging modalities or combined with other super-resolution methods.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628414134

Publication Date

March 11, 2015

Volume

9323
 

Citation

APA
Chicago
ICMJE
MLA
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Yao, J., Wang, L., Li, C., Zhang, C., & Wang, L. V. (2015). Photo-imprint super-resolution photoacoustic microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9323). https://doi.org/10.1117/12.2077350
Yao, J., L. Wang, C. Li, C. Zhang, and L. V. Wang. “Photo-imprint super-resolution photoacoustic microscopy.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 9323, 2015. https://doi.org/10.1117/12.2077350.
Yao J, Wang L, Li C, Zhang C, Wang LV. Photo-imprint super-resolution photoacoustic microscopy. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Yao, J., et al. “Photo-imprint super-resolution photoacoustic microscopy.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9323, 2015. Scopus, doi:10.1117/12.2077350.
Yao J, Wang L, Li C, Zhang C, Wang LV. Photo-imprint super-resolution photoacoustic microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Journal cover image

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628414134

Publication Date

March 11, 2015

Volume

9323