Label-free optical-resolution photoacoustic endomicroscopy in vivo
Publication
, Conference
Yang, JM; Li, C; Chen, R; Rao, B; Yao, J; Yeh, CH; Danielli, A; Maslov, K; Zhou, Q; Shung, KK; Wang, LV
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
March 11, 2015
Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an optical-resolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ∼10 μm at an optical working distance of 6.5 mm. The OR-PAEM system's high-resolution intravital imaging capability is demonstrated through animal experiments.
Duke Scholars
Published In
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
DOI
ISSN
1605-7422
Publication Date
March 11, 2015
Volume
9323
Citation
APA
Chicago
ICMJE
MLA
NLM
Yang, J. M., Li, C., Chen, R., Rao, B., Yao, J., Yeh, C. H., … Wang, L. V. (2015). Label-free optical-resolution photoacoustic endomicroscopy in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9323). https://doi.org/10.1117/12.2080224
Yang, J. M., C. Li, R. Chen, B. Rao, J. Yao, C. H. Yeh, A. Danielli, et al. “Label-free optical-resolution photoacoustic endomicroscopy in vivo.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 9323, 2015. https://doi.org/10.1117/12.2080224.
Yang JM, Li C, Chen R, Rao B, Yao J, Yeh CH, et al. Label-free optical-resolution photoacoustic endomicroscopy in vivo. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Yang, J. M., et al. “Label-free optical-resolution photoacoustic endomicroscopy in vivo.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9323, 2015. Scopus, doi:10.1117/12.2080224.
Yang JM, Li C, Chen R, Rao B, Yao J, Yeh CH, Danielli A, Maslov K, Zhou Q, Shung KK, Wang LV. Label-free optical-resolution photoacoustic endomicroscopy in vivo. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Published In
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
DOI
ISSN
1605-7422
Publication Date
March 11, 2015
Volume
9323