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Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples.

Publication ,  Journal Article
Zhang, YS; Yao, J; Zhang, C; Li, L; Wang, LV; Xia, Y
Published in: Angewandte Chemie (International ed. in English)
July 2014

Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging modality with superb penetration depth and excellent absorption contrast. Here we demonstrate, for the first time, that this technique can advance quantitative analysis of conventional chromogenic histochemistry. Because OR-PAM can quantify the absorption contrast at different wavelengths, it is feasible to spectrally resolve the specific biomolecules involved in a staining color. Furthermore, the tomographic capability of OR-PAM allows for noninvasive volumetric imaging of a thick sample without microtoming it. By immunostaining the sample with different chromogenic agents, we further demonstrated the ability of OR-PAM to resolve different types of cells in a coculture sample with imaging depths up to 1 mm. Taken together, the integration of OR-PAM with (immuno)histochemistry offers a simple and versatile technique with broad applications in cell biology, pathology, tissue engineering, and related biomedical studies.

Duke Scholars

Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

July 2014

Volume

53

Issue

31

Start / End Page

8099 / 8103

Related Subject Headings

  • Spectrophotometry, Ultraviolet
  • Photoacoustic Techniques
  • Organic Chemistry
  • Microscopy
  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

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ICMJE
MLA
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Zhang, Y. S., Yao, J., Zhang, C., Li, L., Wang, L. V., & Xia, Y. (2014). Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples. Angewandte Chemie (International Ed. in English), 53(31), 8099–8103. https://doi.org/10.1002/anie.201403812
Zhang, Yu Shrike, Junjie Yao, Chi Zhang, Lei Li, Lihong V. Wang, and Younan Xia. “Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples.Angewandte Chemie (International Ed. in English) 53, no. 31 (July 2014): 8099–8103. https://doi.org/10.1002/anie.201403812.
Zhang YS, Yao J, Zhang C, Li L, Wang LV, Xia Y. Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples. Angewandte Chemie (International ed in English). 2014 Jul;53(31):8099–103.
Zhang, Yu Shrike, et al. “Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples.Angewandte Chemie (International Ed. in English), vol. 53, no. 31, July 2014, pp. 8099–103. Epmc, doi:10.1002/anie.201403812.
Zhang YS, Yao J, Zhang C, Li L, Wang LV, Xia Y. Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples. Angewandte Chemie (International ed in English). 2014 Jul;53(31):8099–8103.
Journal cover image

Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

July 2014

Volume

53

Issue

31

Start / End Page

8099 / 8103

Related Subject Headings

  • Spectrophotometry, Ultraviolet
  • Photoacoustic Techniques
  • Organic Chemistry
  • Microscopy
  • 34 Chemical sciences
  • 03 Chemical Sciences