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Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy

Publication ,  Conference
Yao, J; Huang, CH; Martel, C; Maslov, KI; Wang, L; Yang, JM; Gao, L; Randolph, G; Zou, J; Wang, LV
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
May 28, 2013

By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena. © 2013 Copyright SPIE.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819493507

Publication Date

May 28, 2013

Volume

8581
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yao, J., Huang, C. H., Martel, C., Maslov, K. I., Wang, L., Yang, J. M., … Wang, L. V. (2013). Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8581). https://doi.org/10.1117/12.2005669
Yao, J., C. H. Huang, C. Martel, K. I. Maslov, L. Wang, J. M. Yang, L. Gao, G. Randolph, J. Zou, and L. V. Wang. “Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 8581, 2013. https://doi.org/10.1117/12.2005669.
Yao J, Huang CH, Martel C, Maslov KI, Wang L, Yang JM, et al. Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2013.
Yao, J., et al. “Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8581, 2013. Scopus, doi:10.1117/12.2005669.
Yao J, Huang CH, Martel C, Maslov KI, Wang L, Yang JM, Gao L, Randolph G, Zou J, Wang LV. Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2013.

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819493507

Publication Date

May 28, 2013

Volume

8581