Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Functional photoacoustic microscopy of diabetic vasculature.

Publication ,  Journal Article
Krumholz, A; Wang, L; Yao, J; Wang, LV
Published in: Journal of biomedical optics
June 2012

We used functional photoacoustic microscopy to image diabetes-induced damage to the microvasculature. To produce an animal model for Type 1 diabetes, we used streptozotocin (STZ), which is particularly toxic to the insulin-producing beta cells of the pancreas in mammals. A set number of ND4 Swiss Webster mice received intraperitoneal injections of STZ for five consecutive days at 50 mg/kg. Most mice developed a significant rise in blood glucose level (≈ 400 mg/dL) within three weeks of the first injection. Changes in vasculature and hemodynamics were monitored for six weeks. The mouse ear was imaged with an optical-resolution photoacoustic microscope at a main blood vessel branch from the root of the ear. There are noticeable and measurable changes associated with the disease, including decreased vessel diameter and possible occlusion due to vessel damage and polyurea. We also observed an increase in the blood flow speed in the vein and a decrease in the artery, which could be due to compensation for the dehydration and vessel diameter changes. Functional and metabolic parameters such as hemoglobin oxygen saturation, oxygen extraction fraction, and oxygen consumption rate were also measured, but showed no significant change.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

June 2012

Volume

17

Issue

6

Start / End Page

060502

Related Subject Headings

  • Streptozocin
  • Polymers
  • Photoacoustic Techniques
  • Oxygen Consumption
  • Oxygen
  • Optics
  • Microscopy
  • Mice
  • Male
  • Light
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Krumholz, A., Wang, L., Yao, J., & Wang, L. V. (2012). Functional photoacoustic microscopy of diabetic vasculature. Journal of Biomedical Optics, 17(6), 060502. https://doi.org/10.1117/1.jbo.17.6.060502
Krumholz, Arie, Lidai Wang, Junjie Yao, and Lihong V. Wang. “Functional photoacoustic microscopy of diabetic vasculature.Journal of Biomedical Optics 17, no. 6 (June 2012): 060502. https://doi.org/10.1117/1.jbo.17.6.060502.
Krumholz A, Wang L, Yao J, Wang LV. Functional photoacoustic microscopy of diabetic vasculature. Journal of biomedical optics. 2012 Jun;17(6):060502.
Krumholz, Arie, et al. “Functional photoacoustic microscopy of diabetic vasculature.Journal of Biomedical Optics, vol. 17, no. 6, June 2012, p. 060502. Epmc, doi:10.1117/1.jbo.17.6.060502.
Krumholz A, Wang L, Yao J, Wang LV. Functional photoacoustic microscopy of diabetic vasculature. Journal of biomedical optics. 2012 Jun;17(6):060502.

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

June 2012

Volume

17

Issue

6

Start / End Page

060502

Related Subject Headings

  • Streptozocin
  • Polymers
  • Photoacoustic Techniques
  • Oxygen Consumption
  • Oxygen
  • Optics
  • Microscopy
  • Mice
  • Male
  • Light