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Functional photoacoustic microscopy of pH

Publication ,  Conference
Chatni, MR; Yao, J; Danielli, A; Favazza, CP; Maslov, KI; Wang, LV
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
February 23, 2012

pH is a tightly regulated indicator of metabolic activity. In mammalian systems, imbalance of pH regulation may result from or result in serious illness. Even though the regulation system of pH is very robust, tissue pH can be altered in many diseases such as cancer, osteoporosis and diabetes mellitus. Traditional high-resolution optical imaging techniques, such as confocal microscopy, routinely image pH in cells and tissues using pH sensitive fluorescent dyes, which change their fluorescence properties with the surrounding pH. Since strong optical scattering in biological tissue blurs images at greater depths, high-resolution pH imaging is limited to penetration depths of 1mm. Here, we report photoacoustic microscopy (PAM) of commercially available pH-sensitive fluorescent dye in tissue phantoms. Using both opticalresolution photoacoustic microscopy (OR-PAM), and acoustic resolution photoacoustic microscopy (AR-PAM), we explored the possibility of recovering the pH values in tissue phantoms. In this paper, we demonstrate that PAM was capable of recovering pH values up to a depth of 2 mm, greater than possible with other forms of optical microscopy. © 2012 SPIE.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819488664

Publication Date

February 23, 2012

Volume

8223
 

Citation

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Chatni, M. R., Yao, J., Danielli, A., Favazza, C. P., Maslov, K. I., & Wang, L. V. (2012). Functional photoacoustic microscopy of pH. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8223). https://doi.org/10.1117/12.907685
Chatni, M. R., J. Yao, A. Danielli, C. P. Favazza, K. I. Maslov, and L. V. Wang. “Functional photoacoustic microscopy of pH.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 8223, 2012. https://doi.org/10.1117/12.907685.
Chatni MR, Yao J, Danielli A, Favazza CP, Maslov KI, Wang LV. Functional photoacoustic microscopy of pH. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2012.
Chatni, M. R., et al. “Functional photoacoustic microscopy of pH.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8223, 2012. Scopus, doi:10.1117/12.907685.
Chatni MR, Yao J, Danielli A, Favazza CP, Maslov KI, Wang LV. Functional photoacoustic microscopy of pH. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2012.
Journal cover image

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819488664

Publication Date

February 23, 2012

Volume

8223