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Photoacoustic microscopy using Evans Blue dye as a contrast agent

Publication ,  Conference
Yao, J; Maslov, KI; Hu, S; Wang, LV
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
February 23, 2010

Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBC) as an endogenous contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we used Evans Blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption at 610 nm and distributes uniformly in the blood stream by chemically binding to albumin. By intravenous injection of EB (6%, 200 μL), complete and continuous microvascular networks-especially capillaries-of the ears of nude mice were imaged. The diffusion of EB (3%, 100 μL) leaving the blood stream was monitored for 2 hours. At lower administration dose of EB (3%, 50 μL), the clearance of the EB-albumin complex was imaged for 10 days and quantitatively investigated using a two-compartment model. © 2010 Copyright SPIE - The International Society for Optical Engineering.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

February 23, 2010

Volume

7564
 

Citation

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Yao, J., Maslov, K. I., Hu, S., & Wang, L. V. (2010). Photoacoustic microscopy using Evans Blue dye as a contrast agent. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7564). https://doi.org/10.1117/12.840684
Yao, J., K. I. Maslov, S. Hu, and L. V. Wang. “Photoacoustic microscopy using Evans Blue dye as a contrast agent.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 7564, 2010. https://doi.org/10.1117/12.840684.
Yao J, Maslov KI, Hu S, Wang LV. Photoacoustic microscopy using Evans Blue dye as a contrast agent. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2010.
Yao, J., et al. “Photoacoustic microscopy using Evans Blue dye as a contrast agent.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7564, 2010. Scopus, doi:10.1117/12.840684.
Yao J, Maslov KI, Hu S, Wang LV. Photoacoustic microscopy using Evans Blue dye as a contrast agent. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2010.

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

February 23, 2010

Volume

7564