Noninvasive quantification of metabolic rate of oxygen (MRO2 ) by photoacoustic microscopy
Many diseases, normal decay and physiological functions are closely related to alterations in the metabolic rate of oxygen (MRO2). In this study, we demonstrate that all the parameters for MRO2 quantification can be simultaneously obtained by optical-resolution photoacoustic microscopy (OR-PAM). MRO2 of the mouse ear under normothermia (31 °C skin temperature) and controlled systematic hyperthermia (42 °C skin temperature) was studied. As a result of hyperthermia, the MRO2 increased by 34.1%. The tumor hypermetabolism was also demonstrated by longitudinally monitoring a melanoma growing on a mouse ear. The results show that OR-PAM, as a single noninvasive imaging modality, is well suited for quantitative MRO 2 measurement in microenvironments. © 2011 SPIE.
