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Photoacoustic microscopy of tyrosinase reporter gene in vivo.

Publication ,  Journal Article
Krumholz, A; Vanvickle-Chavez, SJ; Yao, J; Fleming, TP; Gillanders, WE; Wang, LV
Published in: Journal of biomedical optics
August 2011

Photoacoustic tomography is a hybrid modality based on optical absorption excitation and ultrasonic detection. It is sensitive to melanin, one of the primary absorbers in skin. For cells that do not naturally contain melanin, melanin production can be induced by introducing the gene for tyrosinase, the primary enzyme responsible for expression of melanin in melanogenic cells. Optical resolution photoacoustic microscopy was used in the ex vivo study reported here, where the signal from transfected cells increased by more than 10 times over wild-type cells. A subsequent in vivo experiment was conducted to demonstrate the capability of photoacoustic microscopy to spectrally differentiate between tyrosinase-catalyzed melanin and various other absorbers in tissue.

Duke Scholars

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

August 2011

Volume

16

Issue

8

Start / End Page

080503

Related Subject Headings

  • Ultrasonography
  • Tomography
  • Signal Processing, Computer-Assisted
  • Phantoms, Imaging
  • Oxyhemoglobins
  • Optics
  • Monophenol Monooxygenase
  • Molecular Imaging
  • Microscopy
  • Melanins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Krumholz, A., Vanvickle-Chavez, S. J., Yao, J., Fleming, T. P., Gillanders, W. E., & Wang, L. V. (2011). Photoacoustic microscopy of tyrosinase reporter gene in vivo. Journal of Biomedical Optics, 16(8), 080503. https://doi.org/10.1117/1.3606568
Krumholz, Arie, Sarah J. Vanvickle-Chavez, Junjie Yao, Timothy P. Fleming, William E. Gillanders, and Lihong V. Wang. “Photoacoustic microscopy of tyrosinase reporter gene in vivo.Journal of Biomedical Optics 16, no. 8 (August 2011): 080503. https://doi.org/10.1117/1.3606568.
Krumholz A, Vanvickle-Chavez SJ, Yao J, Fleming TP, Gillanders WE, Wang LV. Photoacoustic microscopy of tyrosinase reporter gene in vivo. Journal of biomedical optics. 2011 Aug;16(8):080503.
Krumholz, Arie, et al. “Photoacoustic microscopy of tyrosinase reporter gene in vivo.Journal of Biomedical Optics, vol. 16, no. 8, Aug. 2011, p. 080503. Epmc, doi:10.1117/1.3606568.
Krumholz A, Vanvickle-Chavez SJ, Yao J, Fleming TP, Gillanders WE, Wang LV. Photoacoustic microscopy of tyrosinase reporter gene in vivo. Journal of biomedical optics. 2011 Aug;16(8):080503.

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

August 2011

Volume

16

Issue

8

Start / End Page

080503

Related Subject Headings

  • Ultrasonography
  • Tomography
  • Signal Processing, Computer-Assisted
  • Phantoms, Imaging
  • Oxyhemoglobins
  • Optics
  • Monophenol Monooxygenase
  • Molecular Imaging
  • Microscopy
  • Melanins