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Boundary cells restrict dystroglycan trafficking to control basement membrane sliding during tissue remodeling.

Publication ,  Journal Article
McClatchey, ST; Wang, Z; Linden, LM; Hastie, EL; Wang, L; Shen, W; Chen, A; Chi, Q; Sherwood, DR
Published in: eLife
September 2016

Epithelial cells and their underlying basement membranes (BMs) slide along each other to renew epithelia, shape organs, and enlarge BM openings. How BM sliding is controlled, however, is poorly understood. Using genetic and live cell imaging approaches during uterine-vulval attachment in C. elegans, we have discovered that the invasive uterine anchor cell activates Notch signaling in neighboring uterine cells at the boundary of the BM gap through which it invades to promote BM sliding. Through an RNAi screen, we found that Notch activation upregulates expression of ctg-1, which encodes a Sec14-GOLD protein, a member of the Sec14 phosphatidylinositol-transfer protein superfamily that is implicated in vesicle trafficking. Through photobleaching, targeted knockdown, and cell-specific rescue, our results suggest that CTG-1 restricts BM adhesion receptor DGN-1 (dystroglycan) trafficking to the cell-BM interface, which promotes BM sliding. Together, these studies reveal a new morphogenetic signaling pathway that controls BM sliding to remodel tissues.

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Published In

eLife

DOI

EISSN

2050-084X

ISSN

2050-084X

Publication Date

September 2016

Volume

5

Start / End Page

e17218

Related Subject Headings

  • Movement
  • Epithelial Cells
  • Dystroglycans
  • Caenorhabditis elegans Proteins
  • Caenorhabditis elegans
  • Basement Membrane
  • Animals
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
 

Citation

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McClatchey, S. T., Wang, Z., Linden, L. M., Hastie, E. L., Wang, L., Shen, W., … Sherwood, D. R. (2016). Boundary cells restrict dystroglycan trafficking to control basement membrane sliding during tissue remodeling. ELife, 5, e17218. https://doi.org/10.7554/elife.17218
McClatchey, Shelly Th, Zheng Wang, Lara M. Linden, Eric L. Hastie, Lin Wang, Wanqing Shen, Alan Chen, Qiuyi Chi, and David R. Sherwood. “Boundary cells restrict dystroglycan trafficking to control basement membrane sliding during tissue remodeling.ELife 5 (September 2016): e17218. https://doi.org/10.7554/elife.17218.
McClatchey ST, Wang Z, Linden LM, Hastie EL, Wang L, Shen W, et al. Boundary cells restrict dystroglycan trafficking to control basement membrane sliding during tissue remodeling. eLife. 2016 Sep;5:e17218.
McClatchey, Shelly Th, et al. “Boundary cells restrict dystroglycan trafficking to control basement membrane sliding during tissue remodeling.ELife, vol. 5, Sept. 2016, p. e17218. Epmc, doi:10.7554/elife.17218.
McClatchey ST, Wang Z, Linden LM, Hastie EL, Wang L, Shen W, Chen A, Chi Q, Sherwood DR. Boundary cells restrict dystroglycan trafficking to control basement membrane sliding during tissue remodeling. eLife. 2016 Sep;5:e17218.

Published In

eLife

DOI

EISSN

2050-084X

ISSN

2050-084X

Publication Date

September 2016

Volume

5

Start / End Page

e17218

Related Subject Headings

  • Movement
  • Epithelial Cells
  • Dystroglycans
  • Caenorhabditis elegans Proteins
  • Caenorhabditis elegans
  • Basement Membrane
  • Animals
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences