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Small choroidal melanoma with chromosome 3 monosomy on fine-needle aspiration biopsy.

Publication ,  Journal Article
Shields, CL; Materin, MA; Teixeira, L; Mashayekhi, A; Ganguly, A; Shields, JA
Published in: Ophthalmology
October 2007

PURPOSE: To evaluate the presence of chromosome 3 monosomy in small choroidal melanoma using fine-needle aspiration biopsy (FNAB). DESIGN: Noncomparative case series. PARTICIPANTS: Fifty-six patients with small choroidal melanoma measuring 3 mm or less in thickness who were undergoing plaque radiotherapy. METHODS: Fine-needle aspiration biopsy was used at the time of plaque radiotherapy to sample tumor cells using a 27-gauge long needle via an indirect transvitreal approach into the tumor apex for postequatorial tumors or a 30-gauge short needle via a direct transscleral approach into the tumor base for preequatorial tumors. MAIN OUTCOME MEASURES: Chromosome 3 monosomy in small choroidal melanoma. RESULTS: The median tumor thickness was 2.6 mm. Monosomy 3 was found in 15 (27%) cases and disomy 3 was found in 32 (57%) cases. In 9 (16%) cases, genomic DNA yield was insufficient for genetic analysis. Fine-needle aspiration biopsy with a 27-gauge needle transvitreal approach provided quantity sufficient for genetic testing in 31 (97%) of 32 cases versus 16 (67%) of 24 cases sampled with a 30-gauge transscleral technique. Compared with disomy 3 tumors, monosomy 3 tumors were statistically more likely to occur in older patients (P = 0.040). Monosomy 3 (versus disomy 3) tumors showed thickness of more than 2 mm in 100% (vs. 84%), subretinal fluid in 87% (vs. 94%), symptoms in 40% (vs. 56%), orange pigment in 93% (vs. 81%), and margin of 3 mm or less to the optic disc in 20% (vs. 50%). There was no statistical difference between monosomy 3 and disomy 3 tumors in the presence or number of these clinical factors. However, small choroidal melanomas with monosomy 3 mutation were more likely to have had documented growth (63%) compared with those with disomy 3 (25%; P = 0.025; odds ratio, 5.00). CONCLUSIONS: Using FNAB at the time of plaque radiotherapy, monosomy 3 was found in approximately 27% of small choroidal melanomas, more often in older patients and tumors with documented growth. Transvitreal biopsy into the tumor apex provided better yield compared with transscleral biopsy into the tumor base.

Duke Scholars

Published In

Ophthalmology

DOI

EISSN

1549-4713

Publication Date

October 2007

Volume

114

Issue

10

Start / End Page

1919 / 1924

Location

United States

Related Subject Headings

  • Risk Factors
  • Ophthalmology & Optometry
  • Monosomy
  • Middle Aged
  • Microsatellite Repeats
  • Melanoma
  • Humans
  • Genetic Techniques
  • DNA, Neoplasm
  • Chromosomes, Human, Pair 3
 

Citation

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Shields, C. L., Materin, M. A., Teixeira, L., Mashayekhi, A., Ganguly, A., & Shields, J. A. (2007). Small choroidal melanoma with chromosome 3 monosomy on fine-needle aspiration biopsy. Ophthalmology, 114(10), 1919–1924. https://doi.org/10.1016/j.ophtha.2007.04.054
Shields, Carol L., Miguel A. Materin, Luiz Teixeira, Arman Mashayekhi, Arupa Ganguly, and Jerry A. Shields. “Small choroidal melanoma with chromosome 3 monosomy on fine-needle aspiration biopsy.Ophthalmology 114, no. 10 (October 2007): 1919–24. https://doi.org/10.1016/j.ophtha.2007.04.054.
Shields CL, Materin MA, Teixeira L, Mashayekhi A, Ganguly A, Shields JA. Small choroidal melanoma with chromosome 3 monosomy on fine-needle aspiration biopsy. Ophthalmology. 2007 Oct;114(10):1919–24.
Shields, Carol L., et al. “Small choroidal melanoma with chromosome 3 monosomy on fine-needle aspiration biopsy.Ophthalmology, vol. 114, no. 10, Oct. 2007, pp. 1919–24. Pubmed, doi:10.1016/j.ophtha.2007.04.054.
Shields CL, Materin MA, Teixeira L, Mashayekhi A, Ganguly A, Shields JA. Small choroidal melanoma with chromosome 3 monosomy on fine-needle aspiration biopsy. Ophthalmology. 2007 Oct;114(10):1919–1924.
Journal cover image

Published In

Ophthalmology

DOI

EISSN

1549-4713

Publication Date

October 2007

Volume

114

Issue

10

Start / End Page

1919 / 1924

Location

United States

Related Subject Headings

  • Risk Factors
  • Ophthalmology & Optometry
  • Monosomy
  • Middle Aged
  • Microsatellite Repeats
  • Melanoma
  • Humans
  • Genetic Techniques
  • DNA, Neoplasm
  • Chromosomes, Human, Pair 3