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Injury-induced ctgfa directs glial bridging and spinal cord regeneration in zebrafish.

Publication ,  Journal Article
Mokalled, MH; Patra, C; Dickson, AL; Endo, T; Stainier, DYR; Poss, KD
Published in: Science
November 4, 2016

Unlike mammals, zebrafish efficiently regenerate functional nervous system tissue after major spinal cord injury. Whereas glial scarring presents a roadblock for mammalian spinal cord repair, glial cells in zebrafish form a bridge across severed spinal cord tissue and facilitate regeneration. We performed a genome-wide profiling screen for secreted factors that are up-regulated during zebrafish spinal cord regeneration. We found that connective tissue growth factor a (ctgfa) is induced in and around glial cells that participate in initial bridging events. Mutations in ctgfa disrupted spinal cord repair, and transgenic ctgfa overexpression or local delivery of human CTGF recombinant protein accelerated bridging and functional regeneration. Our study reveals that CTGF is necessary and sufficient to stimulate glial bridging and natural spinal cord regeneration.

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Published In

Science

DOI

EISSN

1095-9203

Publication Date

November 4, 2016

Volume

354

Issue

6312

Start / End Page

630 / 634

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Spinal Cord Regeneration
  • Spinal Cord Injuries
  • Neuroglia
  • Mutation
  • Male
  • General Science & Technology
  • Female
  • Connective Tissue Growth Factor
 

Citation

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Mokalled, M. H., Patra, C., Dickson, A. L., Endo, T., Stainier, D. Y. R., & Poss, K. D. (2016). Injury-induced ctgfa directs glial bridging and spinal cord regeneration in zebrafish. Science, 354(6312), 630–634. https://doi.org/10.1126/science.aaf2679
Mokalled, Mayssa H., Chinmoy Patra, Amy L. Dickson, Toyokazu Endo, Didier Y. R. Stainier, and Kenneth D. Poss. “Injury-induced ctgfa directs glial bridging and spinal cord regeneration in zebrafish.Science 354, no. 6312 (November 4, 2016): 630–34. https://doi.org/10.1126/science.aaf2679.
Mokalled MH, Patra C, Dickson AL, Endo T, Stainier DYR, Poss KD. Injury-induced ctgfa directs glial bridging and spinal cord regeneration in zebrafish. Science. 2016 Nov 4;354(6312):630–4.
Mokalled, Mayssa H., et al. “Injury-induced ctgfa directs glial bridging and spinal cord regeneration in zebrafish.Science, vol. 354, no. 6312, Nov. 2016, pp. 630–34. Pubmed, doi:10.1126/science.aaf2679.
Mokalled MH, Patra C, Dickson AL, Endo T, Stainier DYR, Poss KD. Injury-induced ctgfa directs glial bridging and spinal cord regeneration in zebrafish. Science. 2016 Nov 4;354(6312):630–634.

Published In

Science

DOI

EISSN

1095-9203

Publication Date

November 4, 2016

Volume

354

Issue

6312

Start / End Page

630 / 634

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Spinal Cord Regeneration
  • Spinal Cord Injuries
  • Neuroglia
  • Mutation
  • Male
  • General Science & Technology
  • Female
  • Connective Tissue Growth Factor