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Muscle protein changes following eccentric exercise in humans.

Publication ,  Journal Article
Reichsman, F; Scordilis, SP; Clarkson, PM; Evans, WJ
Published in: Eur J Appl Physiol Occup Physiol
1991

This study characterized changes in the protein composition of human muscle tissue after eccentric exercise. Four subjects performed 70 maximum eccentric, isokinetic actions of the forearm flexors with one arm. The other arm served as control. A biopsy of the biceps muscle of each arm was taken 2 days after exercise when muscles were very sore (mean = 8.0; 1 = normal; 10 = very, very sore), and muscle damage was documented by a mean decrease of 0.2 radians in the relaxed elbow angle. Proteins from the biopsy tissue were solubilized in a high ionic strength buffer containing several proteolytic inhibitors. Protein concentrations of the extracts were determined and identical amounts loaded onto sodium dodecyl sulfate (SDS) polyacrylamide gels (7.5, 12.5, and 17.5%). Densitometric analysis of the Coomassie brilliant blue stained gels revealed alterations in the amounts of three protein bands in the exercised tissue relative to the control. These changes were in the linear portion of the graph of absorbance versus protein amount. Wilcoxon's signed rank test showed the first two of the following bands to increase significantly in amount (P less than 0.062). The average percentage changes [mean (SEM)] for these bands were 63 (21), 39 (5), and 82 (35). The corresponding molecular weights determined from known standards were 76300 (860), 33200 (310), and 12000 (80) daltons, respectively. These changes imply that the increased synthesis, decreased degradation, or some combination thereof, of these three proteins may be necessary for the repair or regeneration response to exercise-induced muscle damage.

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Published In

Eur J Appl Physiol Occup Physiol

DOI

ISSN

0301-5548

Publication Date

1991

Volume

62

Issue

4

Start / End Page

245 / 250

Location

Germany

Related Subject Headings

  • Sport Sciences
  • Muscles
  • Muscle Proteins
  • Male
  • Humans
  • Female
  • Exercise
  • Arm
 

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Reichsman, F., Scordilis, S. P., Clarkson, P. M., & Evans, W. J. (1991). Muscle protein changes following eccentric exercise in humans. Eur J Appl Physiol Occup Physiol, 62(4), 245–250. https://doi.org/10.1007/BF00571547
Reichsman, F., S. P. Scordilis, P. M. Clarkson, and W. J. Evans. “Muscle protein changes following eccentric exercise in humans.Eur J Appl Physiol Occup Physiol 62, no. 4 (1991): 245–50. https://doi.org/10.1007/BF00571547.
Reichsman F, Scordilis SP, Clarkson PM, Evans WJ. Muscle protein changes following eccentric exercise in humans. Eur J Appl Physiol Occup Physiol. 1991;62(4):245–50.
Reichsman, F., et al. “Muscle protein changes following eccentric exercise in humans.Eur J Appl Physiol Occup Physiol, vol. 62, no. 4, 1991, pp. 245–50. Pubmed, doi:10.1007/BF00571547.
Reichsman F, Scordilis SP, Clarkson PM, Evans WJ. Muscle protein changes following eccentric exercise in humans. Eur J Appl Physiol Occup Physiol. 1991;62(4):245–250.

Published In

Eur J Appl Physiol Occup Physiol

DOI

ISSN

0301-5548

Publication Date

1991

Volume

62

Issue

4

Start / End Page

245 / 250

Location

Germany

Related Subject Headings

  • Sport Sciences
  • Muscles
  • Muscle Proteins
  • Male
  • Humans
  • Female
  • Exercise
  • Arm