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Real-Time Visualization of the Precipitation and Phase Behavior of Octaethylporphyrin in Lipid Microparticles.

Publication ,  Journal Article
Parra, E; Hervella, P; Needham, D
Published in: Journal of pharmaceutical sciences
April 2017

The material properties of micro- and nanoparticles are fundamental for their bulk properties in suspension, like their stability and encapsulation efficiency. A particularly interesting system with potential biomedical applications is the encapsulation of hydrophobic porphyrins into lipid particles and their use as metal atom chelators, where retention and stability are keys for the design process. The overall goal here was to study the solubility, phase behavior, and mixing of octaethylporphyrin (OEP) and OEP-Cu chelates with 2 core materials, triolein (TO) and cholesteryl acetate, as single microparticles. We employed a real-time, single-particle microscopic technique based on micropipette injection to characterize the behavior of these materials and their mixtures upon solvent loss and precipitation. A clear phase separation was observed between the triolein liquid core and porphyrin microcrystals, and the ternary phase diagram of the droplet compositions and onsets of phase separation over solvent dissolution was built. On the contrary, cholesteryl acetate and OEP-Cu coprecipitated by solvent dissolution, preventing porphyrin crystallization even for very high supersaturations. This type of real-time, single-particle characterization is expected to offer important information about the formulation of other hydrophobic compounds of interest, where finding the proper encapsulation environment is a key step for their retention and stability.

Duke Scholars

Published In

Journal of pharmaceutical sciences

DOI

EISSN

1520-6017

ISSN

0022-3549

Publication Date

April 2017

Volume

106

Issue

4

Start / End Page

1025 / 1041

Related Subject Headings

  • Porphyrins
  • Phase Transition
  • Pharmacology & Pharmacy
  • Nanoparticles
  • Lipids
  • Lipid Metabolism
  • Computer Systems
  • Chemical Precipitation
  • 3214 Pharmacology and pharmaceutical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences
 

Citation

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ICMJE
MLA
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Parra, E., Hervella, P., & Needham, D. (2017). Real-Time Visualization of the Precipitation and Phase Behavior of Octaethylporphyrin in Lipid Microparticles. Journal of Pharmaceutical Sciences, 106(4), 1025–1041. https://doi.org/10.1016/j.xphs.2016.11.019
Parra, Elisa, Pablo Hervella, and David Needham. “Real-Time Visualization of the Precipitation and Phase Behavior of Octaethylporphyrin in Lipid Microparticles.Journal of Pharmaceutical Sciences 106, no. 4 (April 2017): 1025–41. https://doi.org/10.1016/j.xphs.2016.11.019.
Parra E, Hervella P, Needham D. Real-Time Visualization of the Precipitation and Phase Behavior of Octaethylporphyrin in Lipid Microparticles. Journal of pharmaceutical sciences. 2017 Apr;106(4):1025–41.
Parra, Elisa, et al. “Real-Time Visualization of the Precipitation and Phase Behavior of Octaethylporphyrin in Lipid Microparticles.Journal of Pharmaceutical Sciences, vol. 106, no. 4, Apr. 2017, pp. 1025–41. Epmc, doi:10.1016/j.xphs.2016.11.019.
Parra E, Hervella P, Needham D. Real-Time Visualization of the Precipitation and Phase Behavior of Octaethylporphyrin in Lipid Microparticles. Journal of pharmaceutical sciences. 2017 Apr;106(4):1025–1041.
Journal cover image

Published In

Journal of pharmaceutical sciences

DOI

EISSN

1520-6017

ISSN

0022-3549

Publication Date

April 2017

Volume

106

Issue

4

Start / End Page

1025 / 1041

Related Subject Headings

  • Porphyrins
  • Phase Transition
  • Pharmacology & Pharmacy
  • Nanoparticles
  • Lipids
  • Lipid Metabolism
  • Computer Systems
  • Chemical Precipitation
  • 3214 Pharmacology and pharmaceutical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences