Hepatic lipogenesis from glucose U 14C and liver dehydrogenases in rats fed Bengal gram (Cicer arietinum) at different protein levels
The effect of feeding defatted Bengal gram (Cicer arietinum) flour at 5, 10 and 20% protein levels respectively to rats for 6 wk was studied on incorporation of glucose U 14C into liver fatty acids and on liver combined hexose monophosphate pathway (HMP) dehydrogenases (EC 1.1.1.44 and EC 1.1.1.49), malate dehydrogenase (decarboxylating) (EC 1.1.1.40), isocitrate dehydrogenase (EC 1.1.1.47) and mitochondrial malate dehydrogenase. The lipogenesis from glucose U 14C was significantly reduced in rats fed Bengal gram at 5 and 10% protein levels as compared to rats fed casein at 20% level. The activity of combined HMP dehydrogenases was reduced in rats fed Bengal gram at 20% protein level and that of malate dehydrogenase (decarboxylating) was increased in rats fed Bengal gram at 5 and 10% protein level as compared to rats fed casein at 20% level.
