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Infectious disease models in zebrafish.

Publication ,  Journal Article
Sullivan, C; Matty, MA; Jurczyszak, D; Gabor, KA; Millard, PJ; Tobin, DM; Kim, CH
Published in: Methods Cell Biol
2017

In recent years, the zebrafish (Danio rerio) has developed as an important alternative to mammalian models for the study of hostpathogen interactions. Because they lack a functional adaptive immune response during the first 4-6weeks of development, zebrafish rely upon innate immune responses to protect against injuries and infections. During this early period of development, it is possible to isolate and study mechanisms of infection and inflammation arising from the innate immune response without the complications presented by the adaptive immune response. Zebrafish possess several inherent characteristics that make them an attractive option to study hostpathogen interactions, including extensive sequence and functional conservation with the human genome, optical clarity in larvae that facilitates the high-resolution visualization of host cell-microbe interactions, a fully sequenced and annotated genome, robust forward and reverse genetic tools and techniques (e.g., CRISPR-Cas9 and TALENs), and amenability to chemical studies and screens. Here, we describe methods for studying hostpathogen interactions both through systemic infections and through localized infections that allow analysis of host cell response, migration patterns, and behavior. Each of the methods described can be modified for use in downstream applications that include ecotoxicant studies and chemical screens.

Duke Scholars

Published In

Methods Cell Biol

DOI

ISSN

0091-679X

Publication Date

2017

Volume

138

Start / End Page

101 / 136

Location

United States

Related Subject Headings

  • Zebrafish
  • Molecular Biology
  • Macrophages
  • Larva
  • Inflammation
  • Infections
  • Immunity, Innate
  • Humans
  • Host-Pathogen Interactions
  • Genome, Human
 

Citation

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ICMJE
MLA
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Sullivan, C., Matty, M. A., Jurczyszak, D., Gabor, K. A., Millard, P. J., Tobin, D. M., & Kim, C. H. (2017). Infectious disease models in zebrafish. Methods Cell Biol, 138, 101–136. https://doi.org/10.1016/bs.mcb.2016.10.005
Sullivan, C., M. A. Matty, D. Jurczyszak, K. A. Gabor, P. J. Millard, D. M. Tobin, and C. H. Kim. “Infectious disease models in zebrafish.Methods Cell Biol 138 (2017): 101–36. https://doi.org/10.1016/bs.mcb.2016.10.005.
Sullivan C, Matty MA, Jurczyszak D, Gabor KA, Millard PJ, Tobin DM, et al. Infectious disease models in zebrafish. Methods Cell Biol. 2017;138:101–36.
Sullivan, C., et al. “Infectious disease models in zebrafish.Methods Cell Biol, vol. 138, 2017, pp. 101–36. Pubmed, doi:10.1016/bs.mcb.2016.10.005.
Sullivan C, Matty MA, Jurczyszak D, Gabor KA, Millard PJ, Tobin DM, Kim CH. Infectious disease models in zebrafish. Methods Cell Biol. 2017;138:101–136.
Journal cover image

Published In

Methods Cell Biol

DOI

ISSN

0091-679X

Publication Date

2017

Volume

138

Start / End Page

101 / 136

Location

United States

Related Subject Headings

  • Zebrafish
  • Molecular Biology
  • Macrophages
  • Larva
  • Inflammation
  • Infections
  • Immunity, Innate
  • Humans
  • Host-Pathogen Interactions
  • Genome, Human