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Ankyrin-rich membrane spanning protein as a novel modulator of transient receptor potential vanilloid 1-function in nociceptive neurons.

Publication ,  Journal Article
Peter, J; Kasper, C; Kaufholz, M; Buschow, R; Isensee, J; Hucho, T; Herberg, FW; Schwede, F; Stein, C; Jordt, S-E; Brackmann, M; Spahn, V
Published in: Eur J Pain
July 2017

BACKGROUND: The ion channel TRPV1 is mainly expressed in small diameter dorsal root ganglion (DRG) neurons, which are involved in the sensation of acute noxious thermal and chemical stimuli. Direct modifications of the channel by diverse signalling events have been intensively investigated, but little is known about the composition of modulating macromolecular TRPV1 signalling complexes. Here, we hypothesize that the novel adaptor protein ankyrin-rich membrane spanning protein/kinase D interacting substrate (ARMS) interacts with TRPV1 and modulates its function in rodent DRG neurons. METHODS: We used immunohistochemistry, electrophysiology, microfluorimetry and immunoprecipitation experiments to investigate TRPV1 and ARMS interactions in DRG neurons and transfected cells. RESULTS: We found that TRPV1 and ARMS are co-expressed in a subpopulation of DRG neurons. ARMS sensitizes TRPV1 towards capsaicin in transfected HEK 293 cells and in mouse DRG neurons in a PKA-dependent manner. Using a combination of functional imaging and immunocytochemistry, we show that the magnitude of the capsaicin response in DRG neurons depends not only on TRPV1 expression, but on the co-expression of ARMS alongside TRPV1. CONCLUSION: These data indicate that ARMS is an important component of the signalling complex regulating the sensitivity of TRPV1. SIGNIFICANCE: The study identifies ARMS as an important component of the signalling complex regulating the sensitivity of excitatory ion channels (TRPV1) in peripheral sensory neurons (DRG neurons) and transfected cells.

Duke Scholars

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Published In

Eur J Pain

DOI

EISSN

1532-2149

Publication Date

July 2017

Volume

21

Issue

6

Start / End Page

1072 / 1086

Location

England

Related Subject Headings

  • TRPV Cation Channels
  • Nociceptors
  • Mice
  • Membrane Proteins
  • Humans
  • HEK293 Cells
  • Ganglia, Spinal
  • Cyclic AMP-Dependent Protein Kinases
  • Capsaicin
  • Animals
 

Citation

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Peter, J., Kasper, C., Kaufholz, M., Buschow, R., Isensee, J., Hucho, T., … Spahn, V. (2017). Ankyrin-rich membrane spanning protein as a novel modulator of transient receptor potential vanilloid 1-function in nociceptive neurons. Eur J Pain, 21(6), 1072–1086. https://doi.org/10.1002/ejp.1008
Peter, J., C. Kasper, M. Kaufholz, R. Buschow, J. Isensee, T. Hucho, F. W. Herberg, et al. “Ankyrin-rich membrane spanning protein as a novel modulator of transient receptor potential vanilloid 1-function in nociceptive neurons.Eur J Pain 21, no. 6 (July 2017): 1072–86. https://doi.org/10.1002/ejp.1008.
Peter J, Kasper C, Kaufholz M, Buschow R, Isensee J, Hucho T, et al. Ankyrin-rich membrane spanning protein as a novel modulator of transient receptor potential vanilloid 1-function in nociceptive neurons. Eur J Pain. 2017 Jul;21(6):1072–86.
Peter, J., et al. “Ankyrin-rich membrane spanning protein as a novel modulator of transient receptor potential vanilloid 1-function in nociceptive neurons.Eur J Pain, vol. 21, no. 6, July 2017, pp. 1072–86. Pubmed, doi:10.1002/ejp.1008.
Peter J, Kasper C, Kaufholz M, Buschow R, Isensee J, Hucho T, Herberg FW, Schwede F, Stein C, Jordt S-E, Brackmann M, Spahn V. Ankyrin-rich membrane spanning protein as a novel modulator of transient receptor potential vanilloid 1-function in nociceptive neurons. Eur J Pain. 2017 Jul;21(6):1072–1086.
Journal cover image

Published In

Eur J Pain

DOI

EISSN

1532-2149

Publication Date

July 2017

Volume

21

Issue

6

Start / End Page

1072 / 1086

Location

England

Related Subject Headings

  • TRPV Cation Channels
  • Nociceptors
  • Mice
  • Membrane Proteins
  • Humans
  • HEK293 Cells
  • Ganglia, Spinal
  • Cyclic AMP-Dependent Protein Kinases
  • Capsaicin
  • Animals