Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Rodent model of reproductive tract leiomyomata. Establishment and characterization of tumor-derived cell lines.

Publication ,  Journal Article
Howe, SR; Gottardis, MM; Everitt, JI; Goldsworthy, TL; Wolf, DC; Walker, C
Published in: Am J Pathol
June 1995

Uterine myometrial tumors are the most commonly found gynecological neoplasm in women. The underlying causes of uterine leiomyomata are poorly understood, a result in part of the absence of a good animal model system in which to study these tumors. This report describes a novel rat model (Eker rat) in which spontaneous gynecological smooth muscle tumors arise with a high frequency. Leiomyomas are the predominant reproductive tract tumor that arise in these animals, although leiomyosarcomas have also been observed. Cell lines have been established from both the benign and malignant lesions. All of the lines express smooth muscle-specific actin, and leiomyoma-derived cell lines express desmin. Two of the cell lines are tumorigenic in nude mice, and the lines are variable for expression of estrogen and progesterone receptors. These lines are the first rodent tumor-derived lines to be established from leiomyomata and are the only lines available from a hereditary form of these tumors. Together with Eker rats that spontaneously develop leiomyomata, they constitute an in vitro/in vivo model system for gaining insights into the mechanism of transformation of uterine smooth muscle cells and the role of steroid hormones and hormone receptors in myometrial tumorigenesis.

Duke Scholars

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

June 1995

Volume

146

Issue

6

Start / End Page

1568 / 1579

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Receptors, Progesterone
  • Receptors, Estrogen
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • RNA
  • Pathology
  • Neoplasm Transplantation
  • Muscle, Smooth
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Howe, S. R., Gottardis, M. M., Everitt, J. I., Goldsworthy, T. L., Wolf, D. C., & Walker, C. (1995). Rodent model of reproductive tract leiomyomata. Establishment and characterization of tumor-derived cell lines. Am J Pathol, 146(6), 1568–1579.
Howe, S. R., M. M. Gottardis, J. I. Everitt, T. L. Goldsworthy, D. C. Wolf, and C. Walker. “Rodent model of reproductive tract leiomyomata. Establishment and characterization of tumor-derived cell lines.Am J Pathol 146, no. 6 (June 1995): 1568–79.
Howe SR, Gottardis MM, Everitt JI, Goldsworthy TL, Wolf DC, Walker C. Rodent model of reproductive tract leiomyomata. Establishment and characterization of tumor-derived cell lines. Am J Pathol. 1995 Jun;146(6):1568–79.
Howe, S. R., et al. “Rodent model of reproductive tract leiomyomata. Establishment and characterization of tumor-derived cell lines.Am J Pathol, vol. 146, no. 6, June 1995, pp. 1568–79.
Howe SR, Gottardis MM, Everitt JI, Goldsworthy TL, Wolf DC, Walker C. Rodent model of reproductive tract leiomyomata. Establishment and characterization of tumor-derived cell lines. Am J Pathol. 1995 Jun;146(6):1568–1579.
Journal cover image

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

June 1995

Volume

146

Issue

6

Start / End Page

1568 / 1579

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Receptors, Progesterone
  • Receptors, Estrogen
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • RNA
  • Pathology
  • Neoplasm Transplantation
  • Muscle, Smooth