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Six1 and Six1 cofactor expression is altered during early skeletal muscle overload in mice.

Publication ,  Journal Article
Gordon, BS; Delgado Díaz, DC; White, JP; Carson, JA; Kostek, MC
Published in: J Physiol Sci
September 2012

Six1 is a transcription factor that, along with cofactors (Eya1, Eya3, and Dach2), regulates skeletal muscle fiber-type and development. SIX1 (human) gene expression decreases after overload, but the time course of Six1 expression, if protein is affected, and if the response differs between muscles with differing phenotypes, is not known. Our purpose was to examine Six1 gene and protein expression and co-factor gene expression during the initiation of muscle overload, and determine if the muscle phenotype altered this response. The plantaris and soleus were functionally overloaded by synergistic ablation of the gastrocnemius, and Six1 gene and protein, and Six1 cofactor gene expression was measured. Six1 gene expression decreased at 1 day of overload 48 ± 9 and 47 ± 20 % (p < 0.01) in the plantaris and soleus. After 3 days of overload, Six1 protein expression increased 73 ± 17 and 168 ± 57 % in the plantaris and soleus (p < 0.05). After 1 day of overload, Dach2 gene expression decreased 56 ± 9 and 35 ± 3 % in both muscles (p < 0.001), while Eya1 decreased 33 ± 5 % only in the soleus (p < 0.01). Eya3 gene expression increased 127 ± 26 % (p < 0.05) and 76 ± 16 % (p < 0.05) in the plantaris and soleus, while Dach2 gene expression decreased 71 ± 4 % (p < 0.05) in the soleus after 3 days of overload. Six1 and Six1 co-factor expression is responsive to muscle overload in both fast and slow muscles. This indicates that this molecular program may affect overload adaptation regardless of muscle phenotype.

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Published In

J Physiol Sci

DOI

EISSN

1880-6562

Publication Date

September 2012

Volume

62

Issue

5

Start / End Page

393 / 401

Location

Japan

Related Subject Headings

  • Transcription Factors
  • Protein Tyrosine Phosphatases
  • Physiology
  • Nuclear Proteins
  • Muscle, Skeletal
  • Muscle Fibers, Slow-Twitch
  • Muscle Fibers, Fast-Twitch
  • Mice, Inbred C57BL
  • Mice
  • Male
 

Citation

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Gordon, B. S., Delgado Díaz, D. C., White, J. P., Carson, J. A., & Kostek, M. C. (2012). Six1 and Six1 cofactor expression is altered during early skeletal muscle overload in mice. J Physiol Sci, 62(5), 393–401. https://doi.org/10.1007/s12576-012-0214-y
Gordon, Bradley S., Diana C. Delgado Díaz, James P. White, James A. Carson, and Matthew C. Kostek. “Six1 and Six1 cofactor expression is altered during early skeletal muscle overload in mice.J Physiol Sci 62, no. 5 (September 2012): 393–401. https://doi.org/10.1007/s12576-012-0214-y.
Gordon BS, Delgado Díaz DC, White JP, Carson JA, Kostek MC. Six1 and Six1 cofactor expression is altered during early skeletal muscle overload in mice. J Physiol Sci. 2012 Sep;62(5):393–401.
Gordon, Bradley S., et al. “Six1 and Six1 cofactor expression is altered during early skeletal muscle overload in mice.J Physiol Sci, vol. 62, no. 5, Sept. 2012, pp. 393–401. Pubmed, doi:10.1007/s12576-012-0214-y.
Gordon BS, Delgado Díaz DC, White JP, Carson JA, Kostek MC. Six1 and Six1 cofactor expression is altered during early skeletal muscle overload in mice. J Physiol Sci. 2012 Sep;62(5):393–401.
Journal cover image

Published In

J Physiol Sci

DOI

EISSN

1880-6562

Publication Date

September 2012

Volume

62

Issue

5

Start / End Page

393 / 401

Location

Japan

Related Subject Headings

  • Transcription Factors
  • Protein Tyrosine Phosphatases
  • Physiology
  • Nuclear Proteins
  • Muscle, Skeletal
  • Muscle Fibers, Slow-Twitch
  • Muscle Fibers, Fast-Twitch
  • Mice, Inbred C57BL
  • Mice
  • Male