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Differences in Reperfusion-Induced Mitochondrial Oxidative Stress and Cell Death Between Hippocampal CA1 and CA3 Subfields Are Due to the Mitochondrial Thioredoxin System.

Publication ,  Journal Article
Yin, B; Barrionuevo, G; Batinic-Haberle, I; Sandberg, M; Weber, SG
Published in: Antioxid Redox Signal
September 20, 2017

AIMS: The susceptibility of CA1 over CA3 to damage from cerebral ischemia may be related to the differences in reactive oxygen species (ROS) production/removal between the two hippocampal subfields. We aimed to measure CA1/CA3 differences in net ROS production in real time in the first 30 min of reperfusion in pyramidal cells. We aimed to determine the underlying cause of the differential vulnerability of CA1 and CA3. RESULTS: Real-time determinations of mitochondrial H2O2 and, independently, glutathione (GSH) redox status from roGFP-based probes in individual pyramidal cells in organotypic hippocampal cultures during oxygen-glucose deprivation (OGD)-reperfusion (RP) demonstrate a significantly more oxidizing environment during RP in CA1 than CA3 mitochondria. Protein levels (immunohistochemistry and Western blots), roGFP2-based probe measurements during controlled mitochondrial production of ROS, and thioredoxin reductase (TrxR) inhibition by auranofin are consistent with a more effective mitochondrial thioredoxin (Trx) system in CA3. Inhibition of TrxR eliminates the differences in redox status and cell death between the regions. Overexpression of cytosolic Trx1 does not influence mitochondrial H2O2 production. INNOVATION: Real-time changes of mitochondrial H2O2 and GSH in tissue cultures during early RP, and also during controlled production of superoxide and peroxide, reveal significant differences between CA1 and CA3. The mitochondrial Trx system is responsible for the observed differences during RP as well as for delayed cell death 18 h afterward. CONCLUSION: Greater mitochondrial Trx efficacy in CA3 pyramidal cells results in less vulnerability to ischemia/reperfusion because of the less oxidizing environment in CA3 mitochondria during RP. Antioxid. Redox Signal. 27, 534-549.

Duke Scholars

Published In

Antioxid Redox Signal

DOI

EISSN

1557-7716

Publication Date

September 20, 2017

Volume

27

Issue

9

Start / End Page

534 / 549

Location

United States

Related Subject Headings

  • Thioredoxin-Disulfide Reductase
  • Reperfusion Injury
  • Reactive Oxygen Species
  • Rats
  • Oxidative Stress
  • Organ Culture Techniques
  • Mitochondria
  • Male
  • Hydrogen Peroxide
  • Hippocampus
 

Citation

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Yin, B., Barrionuevo, G., Batinic-Haberle, I., Sandberg, M., & Weber, S. G. (2017). Differences in Reperfusion-Induced Mitochondrial Oxidative Stress and Cell Death Between Hippocampal CA1 and CA3 Subfields Are Due to the Mitochondrial Thioredoxin System. Antioxid Redox Signal, 27(9), 534–549. https://doi.org/10.1089/ars.2016.6706
Yin, Bocheng, Germán Barrionuevo, Ines Batinic-Haberle, Mats Sandberg, and Stephen G. Weber. “Differences in Reperfusion-Induced Mitochondrial Oxidative Stress and Cell Death Between Hippocampal CA1 and CA3 Subfields Are Due to the Mitochondrial Thioredoxin System.Antioxid Redox Signal 27, no. 9 (September 20, 2017): 534–49. https://doi.org/10.1089/ars.2016.6706.
Yin B, Barrionuevo G, Batinic-Haberle I, Sandberg M, Weber SG. Differences in Reperfusion-Induced Mitochondrial Oxidative Stress and Cell Death Between Hippocampal CA1 and CA3 Subfields Are Due to the Mitochondrial Thioredoxin System. Antioxid Redox Signal. 2017 Sep 20;27(9):534–49.
Yin, Bocheng, et al. “Differences in Reperfusion-Induced Mitochondrial Oxidative Stress and Cell Death Between Hippocampal CA1 and CA3 Subfields Are Due to the Mitochondrial Thioredoxin System.Antioxid Redox Signal, vol. 27, no. 9, Sept. 2017, pp. 534–49. Pubmed, doi:10.1089/ars.2016.6706.
Yin B, Barrionuevo G, Batinic-Haberle I, Sandberg M, Weber SG. Differences in Reperfusion-Induced Mitochondrial Oxidative Stress and Cell Death Between Hippocampal CA1 and CA3 Subfields Are Due to the Mitochondrial Thioredoxin System. Antioxid Redox Signal. 2017 Sep 20;27(9):534–549.
Journal cover image

Published In

Antioxid Redox Signal

DOI

EISSN

1557-7716

Publication Date

September 20, 2017

Volume

27

Issue

9

Start / End Page

534 / 549

Location

United States

Related Subject Headings

  • Thioredoxin-Disulfide Reductase
  • Reperfusion Injury
  • Reactive Oxygen Species
  • Rats
  • Oxidative Stress
  • Organ Culture Techniques
  • Mitochondria
  • Male
  • Hydrogen Peroxide
  • Hippocampus