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Galectin-3 directs antimicrobial guanylate binding proteins to vacuoles furnished with bacterial secretion systems.

Publication ,  Journal Article
Feeley, EM; Pilla-Moffett, DM; Zwack, EE; Piro, AS; Finethy, R; Kolb, JP; Martinez, J; Brodsky, IE; Coers, J
Published in: Proc Natl Acad Sci U S A
February 28, 2017

Many invasive bacteria establish pathogen-containing vacuoles (PVs) as intracellular niches for microbial growth. Immunity to these infections is dependent on the ability of host cells to recognize PVs as targets for host defense. The delivery of several host defense proteins to PVs is controlled by IFN-inducible guanylate binding proteins (GBPs), which themselves dock to PVs through poorly characterized mechanisms. Here, we demonstrate that GBPs detect the presence of bacterial protein secretion systems as "patterns of pathogenesis" associated with PVs. We report that the delivery of GBP2 to Legionella-containing vacuoles is dependent on the bacterial Dot/Icm secretion system, whereas the delivery of GBP2 to Yersinia-containing vacuoles (YCVs) requires hypersecretion of Yersinia translocon proteins. We show that the presence of bacterial secretion systems directs cytosolic carbohydrate-binding protein Galectin-3 to PVs and that the delivery of GBP1 and GBP2 to Legionella-containing vacuoles or YCVs is substantially diminished in Galectin-3-deficient cells. Our results illustrate that insertion of bacterial secretion systems into PV membranes stimulates Galectin-3-dependent recruitment of antimicrobial GBPs to PVs as part of a coordinated host defense program.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

February 28, 2017

Volume

114

Issue

9

Start / End Page

E1698 / E1706

Location

United States

Related Subject Headings

  • Vacuoles
  • Receptors, Cell Surface
  • RAW 264.7 Cells
  • Mice, Inbred C57BL
  • Mice
  • Legionella
  • Humans
  • HEK293 Cells
  • Galectin 3
  • GTP-Binding Proteins
 

Citation

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Feeley, E. M., Pilla-Moffett, D. M., Zwack, E. E., Piro, A. S., Finethy, R., Kolb, J. P., … Coers, J. (2017). Galectin-3 directs antimicrobial guanylate binding proteins to vacuoles furnished with bacterial secretion systems. Proc Natl Acad Sci U S A, 114(9), E1698–E1706. https://doi.org/10.1073/pnas.1615771114
Feeley, Eric M., Danielle M. Pilla-Moffett, Erin E. Zwack, Anthony S. Piro, Ryan Finethy, Joseph P. Kolb, Jennifer Martinez, Igor E. Brodsky, and Jörn Coers. “Galectin-3 directs antimicrobial guanylate binding proteins to vacuoles furnished with bacterial secretion systems.Proc Natl Acad Sci U S A 114, no. 9 (February 28, 2017): E1698–1706. https://doi.org/10.1073/pnas.1615771114.
Feeley EM, Pilla-Moffett DM, Zwack EE, Piro AS, Finethy R, Kolb JP, et al. Galectin-3 directs antimicrobial guanylate binding proteins to vacuoles furnished with bacterial secretion systems. Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):E1698–706.
Feeley, Eric M., et al. “Galectin-3 directs antimicrobial guanylate binding proteins to vacuoles furnished with bacterial secretion systems.Proc Natl Acad Sci U S A, vol. 114, no. 9, Feb. 2017, pp. E1698–706. Pubmed, doi:10.1073/pnas.1615771114.
Feeley EM, Pilla-Moffett DM, Zwack EE, Piro AS, Finethy R, Kolb JP, Martinez J, Brodsky IE, Coers J. Galectin-3 directs antimicrobial guanylate binding proteins to vacuoles furnished with bacterial secretion systems. Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):E1698–E1706.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

February 28, 2017

Volume

114

Issue

9

Start / End Page

E1698 / E1706

Location

United States

Related Subject Headings

  • Vacuoles
  • Receptors, Cell Surface
  • RAW 264.7 Cells
  • Mice, Inbred C57BL
  • Mice
  • Legionella
  • Humans
  • HEK293 Cells
  • Galectin 3
  • GTP-Binding Proteins