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Caspofungin exposure alters the core septin AspB interactome of Aspergillus fumigatus.

Publication ,  Journal Article
Vargas-Muñiz, JM; Renshaw, H; Waitt, G; Soderblom, EJ; Moseley, MA; Palmer, JM; Juvvadi, PR; Keller, NP; Steinbach, WJ
Published in: Biochem Biophys Res Commun
April 1, 2017

Aspergillus fumigatus, the main etiological agent of invasive aspergillosis, is a leading cause of death in immunocompromised patients. Septins, a conserved family of GTP-binding proteins, serve as scaffolding proteins to recruit enzymes and key regulators to different cellular compartments. Deletion of the A. fumigatus septin aspB increases susceptibility to the echinocandin antifungal caspofungin. However, how AspB mediates this response to caspofungin is unknown. Here, we characterized the AspB interactome under basal conditions and after exposure to a clinically relevant concentration of caspofungin. While A. fumigatus AspB interacted with 334 proteins, including kinases, cell cycle regulators, and cell wall synthesis-related proteins under basal growth conditions, caspofungin exposure altered AspB interactions. A total of 69 of the basal interactants did not interact with AspB after exposure to caspofungin, and 54 new interactants were identified following caspofungin exposure. We generated A. fumigatus deletion strains for 3 proteins (ArpB, Cyp4, and PpoA) that only interacted with AspB following exposure to caspofungin that were previously annotated as induced after exposure to antifungal agents, yet only PpoA was implicated in the response to caspofungin. Taken together, we defined how the septin AspB interactome is altered in the presence of a clinically relevant antifungal.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

April 1, 2017

Volume

485

Issue

2

Start / End Page

221 / 226

Location

United States

Related Subject Headings

  • Septins
  • Protein Interaction Maps
  • Lipopeptides
  • Humans
  • Gene Deletion
  • Fungal Proteins
  • Echinocandins
  • Caspofungin
  • Biochemistry & Molecular Biology
  • Aspergillus fumigatus
 

Citation

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Vargas-Muñiz, J. M., Renshaw, H., Waitt, G., Soderblom, E. J., Moseley, M. A., Palmer, J. M., … Steinbach, W. J. (2017). Caspofungin exposure alters the core septin AspB interactome of Aspergillus fumigatus. Biochem Biophys Res Commun, 485(2), 221–226. https://doi.org/10.1016/j.bbrc.2017.02.116
Vargas-Muñiz, José M., Hilary Renshaw, Greg Waitt, Erik J. Soderblom, M Arthur Moseley, Jonathan M. Palmer, Praveen R. Juvvadi, Nancy P. Keller, and William J. Steinbach. “Caspofungin exposure alters the core septin AspB interactome of Aspergillus fumigatus.Biochem Biophys Res Commun 485, no. 2 (April 1, 2017): 221–26. https://doi.org/10.1016/j.bbrc.2017.02.116.
Vargas-Muñiz JM, Renshaw H, Waitt G, Soderblom EJ, Moseley MA, Palmer JM, et al. Caspofungin exposure alters the core septin AspB interactome of Aspergillus fumigatus. Biochem Biophys Res Commun. 2017 Apr 1;485(2):221–6.
Vargas-Muñiz, José M., et al. “Caspofungin exposure alters the core septin AspB interactome of Aspergillus fumigatus.Biochem Biophys Res Commun, vol. 485, no. 2, Apr. 2017, pp. 221–26. Pubmed, doi:10.1016/j.bbrc.2017.02.116.
Vargas-Muñiz JM, Renshaw H, Waitt G, Soderblom EJ, Moseley MA, Palmer JM, Juvvadi PR, Keller NP, Steinbach WJ. Caspofungin exposure alters the core septin AspB interactome of Aspergillus fumigatus. Biochem Biophys Res Commun. 2017 Apr 1;485(2):221–226.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

April 1, 2017

Volume

485

Issue

2

Start / End Page

221 / 226

Location

United States

Related Subject Headings

  • Septins
  • Protein Interaction Maps
  • Lipopeptides
  • Humans
  • Gene Deletion
  • Fungal Proteins
  • Echinocandins
  • Caspofungin
  • Biochemistry & Molecular Biology
  • Aspergillus fumigatus