Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.
Publication
, Journal Article
Ghysdael, J; Hubert, E; Trávnícek, M; Bolognesi, DP; Burny, A; Cleuter, Y; Huez, G; Kettmann, R; Marbaix, G; Portetelle, D; Chantrenne, H
Published in: Proc Natl Acad Sci U S A
August 1977
After microinjection of Xenopus laevis oocytes with RNA from avian myeloblastosis virus, viral structural proteins p27, p19, p15, and p12 are formed by a sequence of posttranslational cleavages of a high-molecular-weight precursor polypeptide. The 60-70S RNA aggregate or its 30-40S RNA subunits obtained by heat or formamide treatment possess the same ability to serve as template in X. laevis oocytes. The processing pattern of virus-specific precursor polypeptides is the same in X. laevis oocytes as in chick embryo fibroblasts infected with avian myeloblastosis virus, but the processing takes place at a much slower rate.
Duke Scholars
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
August 1977
Volume
74
Issue
8
Start / End Page
3230 / 3234
Location
United States
Related Subject Headings
- Xenopus
- Viral Proteins
- RNA, Viral
- Protein Biosynthesis
- Precipitin Tests
- Peptide Biosynthesis
- Ovum
- Oocytes
- Molecular Weight
- Microinjections
Citation
APA
Chicago
ICMJE
MLA
NLM
Ghysdael, J., Hubert, E., Trávnícek, M., Bolognesi, D. P., Burny, A., Cleuter, Y., … Chantrenne, H. (1977). Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA. Proc Natl Acad Sci U S A, 74(8), 3230–3234. https://doi.org/10.1073/pnas.74.8.3230
Ghysdael, J., E. Hubert, M. Trávnícek, D. P. Bolognesi, A. Burny, Y. Cleuter, G. Huez, et al. “Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.” Proc Natl Acad Sci U S A 74, no. 8 (August 1977): 3230–34. https://doi.org/10.1073/pnas.74.8.3230.
Ghysdael J, Hubert E, Trávnícek M, Bolognesi DP, Burny A, Cleuter Y, et al. Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3230–4.
Ghysdael, J., et al. “Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.” Proc Natl Acad Sci U S A, vol. 74, no. 8, Aug. 1977, pp. 3230–34. Pubmed, doi:10.1073/pnas.74.8.3230.
Ghysdael J, Hubert E, Trávnícek M, Bolognesi DP, Burny A, Cleuter Y, Huez G, Kettmann R, Marbaix G, Portetelle D, Chantrenne H. Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3230–3234.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
August 1977
Volume
74
Issue
8
Start / End Page
3230 / 3234
Location
United States
Related Subject Headings
- Xenopus
- Viral Proteins
- RNA, Viral
- Protein Biosynthesis
- Precipitin Tests
- Peptide Biosynthesis
- Ovum
- Oocytes
- Molecular Weight
- Microinjections