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Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats.

Publication ,  Journal Article
Kelly, LW; Barden, CA; Tiedeman, JS; Hatchell, DL
Published in: Exp Eye Res
March 1993

Capillary closure and venous dilatation occur early in diabetic retinopathy, and altered blood rheology may play a role. For example previous studies have shown leukocytes are less deformable, are more activated, and occlude retinal capillaries more often in diabetic subjects. The purpose of this study was to determine if rheologic changes developed in diabetic cats, a model in which we have documented retinal capillary basement membrane thickening, microaneurysms, and intraretinal hemorrhage characteristics of early diabetic retinopathy. Viscosity of blood, plasma and purified erythrocyte suspensions was measured by rotational viscometry and plasma fibrinogen content was determined by heat precipitation. Filterability of blood and purified erythrocyte, mononuclear leukocyte, and granulocyte suspensions was determined at constant flow, measuring the increase in pressure over 2 min relative to the pressure generated by buffer alone. Viscosity of whole blood and plasma, but not erythrocytes, was significantly elevated (P < 0.005) in the diabetic cats over normals at all shear rates studied (450, 225 and 90 sec-1). Furthermore, fibrinogen levels were significantly elevated in diabetic cats compared to normals (P < 0.004), and were positively correlated with the viscosity of whole blood and plasma. The filterability of mononuclear leukocytes and whole blood in diabetic cats was decreased 56% and 74% when compared to normals, P < 0.0006 and P < 0.025, respectively. In contrast, the filterability of granulocytes and erythrocytes was not significantly different between the two groups. These results suggest that the rheologic alterations in diabetes are numerous, and involve both cellular and plasma protein changes.(ABSTRACT TRUNCATED AT 250 WORDS)

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Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

March 1993

Volume

56

Issue

3

Start / End Page

341 / 347

Location

England

Related Subject Headings

  • Plasma
  • Ophthalmology & Optometry
  • Microscopy, Electron, Scanning
  • Granulocytes
  • Fibrinogen
  • Erythrocyte Deformability
  • Diabetic Retinopathy
  • Diabetes Mellitus, Experimental
  • Cats
  • Blood Viscosity
 

Citation

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Kelly, L. W., Barden, C. A., Tiedeman, J. S., & Hatchell, D. L. (1993). Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats. Exp Eye Res, 56(3), 341–347. https://doi.org/10.1006/exer.1993.1044
Kelly, L. W., C. A. Barden, J. S. Tiedeman, and D. L. Hatchell. “Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats.Exp Eye Res 56, no. 3 (March 1993): 341–47. https://doi.org/10.1006/exer.1993.1044.
Kelly LW, Barden CA, Tiedeman JS, Hatchell DL. Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats. Exp Eye Res. 1993 Mar;56(3):341–7.
Kelly, L. W., et al. “Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats.Exp Eye Res, vol. 56, no. 3, Mar. 1993, pp. 341–47. Pubmed, doi:10.1006/exer.1993.1044.
Kelly LW, Barden CA, Tiedeman JS, Hatchell DL. Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats. Exp Eye Res. 1993 Mar;56(3):341–347.
Journal cover image

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

March 1993

Volume

56

Issue

3

Start / End Page

341 / 347

Location

England

Related Subject Headings

  • Plasma
  • Ophthalmology & Optometry
  • Microscopy, Electron, Scanning
  • Granulocytes
  • Fibrinogen
  • Erythrocyte Deformability
  • Diabetic Retinopathy
  • Diabetes Mellitus, Experimental
  • Cats
  • Blood Viscosity