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Ki-67, p53, and p16 expression, and G691S RET polymorphism in desmoplastic melanoma (DM): A clinicopathologic analysis of predictors of outcome.

Publication ,  Journal Article
Lawrence, NF; Hammond, MR; Frederick, DT; Su, Y; Dias-Santagata, D; Deng, A; Selim, MA; Mahalingam, M; Flaherty, KT; Hoang, MP
Published in: J Am Acad Dermatol
September 2016

BACKGROUND: The prognostic role Ki-67, p53, and p16 immunostains and RET (rearranged during transfection) polymorphism in desmoplastic melanoma has not been evaluated. OBJECTIVE: We sought to identify potential prognostic markers. METHODS: We performed Ki-67, p53, and p16 immunostains on 66 desmoplastic melanomas, and sequenced RET G691 polymorphism and recurrent mutations of 17 cancer genes in 55 and 20 cases, respectively. RESULTS: Recurrence and metastasis were documented in 11 of 66 (17%) and 26 of 66 (39%) patients, respectively. Death was noted in 25 of 55 (45%) patients. Ki-67 expression (≥10%, 43%) correlated with male gender (P = .009), ulceration (P = .002), and Breslow depth (P = .009). p53 Expression (≥50%, 28%) correlated with male gender (P = .002) and head and neck location (P = .0228). Using Kaplan-Meier plots, Ki-67 expression (P = .0425) and mitosis (P = .00295) correlated with overall survival, whereas vascular invasion (P = .0292) correlated with disease progression. There was a significant correlation between Ki-67 and p53 expression (P = .003). RET polymorphism was present in 10 of 46 (22%) cases and inversely correlated with Breslow depth (P = .024). LIMITATION: Our study is small and lacks power to perform a multivariate analysis. CONCLUSION: Although Ki-67 expression correlated with overall survival, additional studies are needed to determine whether Ki-67 would be an independent prognostic marker in addition to the current routine histopathologic assessment.

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Published In

J Am Acad Dermatol

DOI

EISSN

1097-6787

Publication Date

September 2016

Volume

75

Issue

3

Start / End Page

595 / 602

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Survival Analysis
  • Skin Neoplasms
  • Proto-Oncogene Proteins c-ret
  • Prospective Studies
  • Prognosis
  • Predictive Value of Tests
  • Polymorphism, Genetic
  • Middle Aged
  • Melanoma, Cutaneous Malignant
 

Citation

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Lawrence, N. F., Hammond, M. R., Frederick, D. T., Su, Y., Dias-Santagata, D., Deng, A., … Hoang, M. P. (2016). Ki-67, p53, and p16 expression, and G691S RET polymorphism in desmoplastic melanoma (DM): A clinicopathologic analysis of predictors of outcome. J Am Acad Dermatol, 75(3), 595–602. https://doi.org/10.1016/j.jaad.2016.04.059
Lawrence, Nicholas F., Marc R. Hammond, Dennie T. Frederick, Yuhua Su, Dora Dias-Santagata, April Deng, M Angelica Selim, Meera Mahalingam, Keith T. Flaherty, and Mai P. Hoang. “Ki-67, p53, and p16 expression, and G691S RET polymorphism in desmoplastic melanoma (DM): A clinicopathologic analysis of predictors of outcome.J Am Acad Dermatol 75, no. 3 (September 2016): 595–602. https://doi.org/10.1016/j.jaad.2016.04.059.
Lawrence NF, Hammond MR, Frederick DT, Su Y, Dias-Santagata D, Deng A, et al. Ki-67, p53, and p16 expression, and G691S RET polymorphism in desmoplastic melanoma (DM): A clinicopathologic analysis of predictors of outcome. J Am Acad Dermatol. 2016 Sep;75(3):595–602.
Lawrence, Nicholas F., et al. “Ki-67, p53, and p16 expression, and G691S RET polymorphism in desmoplastic melanoma (DM): A clinicopathologic analysis of predictors of outcome.J Am Acad Dermatol, vol. 75, no. 3, Sept. 2016, pp. 595–602. Pubmed, doi:10.1016/j.jaad.2016.04.059.
Lawrence NF, Hammond MR, Frederick DT, Su Y, Dias-Santagata D, Deng A, Selim MA, Mahalingam M, Flaherty KT, Hoang MP. Ki-67, p53, and p16 expression, and G691S RET polymorphism in desmoplastic melanoma (DM): A clinicopathologic analysis of predictors of outcome. J Am Acad Dermatol. 2016 Sep;75(3):595–602.
Journal cover image

Published In

J Am Acad Dermatol

DOI

EISSN

1097-6787

Publication Date

September 2016

Volume

75

Issue

3

Start / End Page

595 / 602

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Survival Analysis
  • Skin Neoplasms
  • Proto-Oncogene Proteins c-ret
  • Prospective Studies
  • Prognosis
  • Predictive Value of Tests
  • Polymorphism, Genetic
  • Middle Aged
  • Melanoma, Cutaneous Malignant