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Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration.

Publication ,  Journal Article
Pearring, JN; San Agustin, JT; Lobanova, ES; Gabriel, CJ; Lieu, EC; Monis, WJ; Stuck, MW; Strittmatter, L; Jaber, SM; Arshavsky, VY; Pazour, GJ
Published in: PLoS Genet
April 2017

Arf4 is proposed to be a critical regulator of membrane protein trafficking in early secretory pathway. More recently, Arf4 was also implicated in regulating ciliary trafficking, however, this has not been comprehensively tested in vivo. To directly address Arf4's role in ciliary transport, we deleted Arf4 specifically in either rod photoreceptor cells, kidney, or globally during the early postnatal period. Arf4 deletion in photoreceptors did not cause protein mislocalization or retinal degeneration, as expected if Arf4 played a role in protein transport to the ciliary outer segment. Likewise, Arf4 deletion in kidney did not cause cystic disease, as expected if Arf4 were involved in general ciliary trafficking. In contrast, global Arf4 deletion in the early postnatal period resulted in growth restriction, severe pancreatic degeneration and early death. These findings are consistent with Arf4 playing a critical role in endomembrane trafficking, particularly in the pancreas, but not in ciliary function.

Duke Scholars

Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

April 2017

Volume

13

Issue

4

Start / End Page

e1006740

Location

United States

Related Subject Headings

  • Sequence Deletion
  • Retinal Degeneration
  • Photoreceptor Cells
  • Pancreas, Exocrine
  • Nucleotide Motifs
  • Mice, Knockout
  • Mice
  • Kidney Diseases, Cystic
  • Kidney
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pearring, J. N., San Agustin, J. T., Lobanova, E. S., Gabriel, C. J., Lieu, E. C., Monis, W. J., … Pazour, G. J. (2017). Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration. PLoS Genet, 13(4), e1006740. https://doi.org/10.1371/journal.pgen.1006740
Pearring, Jillian N., Jovenal T. San Agustin, Ekaterina S. Lobanova, Christopher J. Gabriel, Eric C. Lieu, William J. Monis, Michael W. Stuck, et al. “Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration.PLoS Genet 13, no. 4 (April 2017): e1006740. https://doi.org/10.1371/journal.pgen.1006740.
Pearring JN, San Agustin JT, Lobanova ES, Gabriel CJ, Lieu EC, Monis WJ, et al. Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration. PLoS Genet. 2017 Apr;13(4):e1006740.
Pearring, Jillian N., et al. “Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration.PLoS Genet, vol. 13, no. 4, Apr. 2017, p. e1006740. Pubmed, doi:10.1371/journal.pgen.1006740.
Pearring JN, San Agustin JT, Lobanova ES, Gabriel CJ, Lieu EC, Monis WJ, Stuck MW, Strittmatter L, Jaber SM, Arshavsky VY, Pazour GJ. Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration. PLoS Genet. 2017 Apr;13(4):e1006740.

Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

April 2017

Volume

13

Issue

4

Start / End Page

e1006740

Location

United States

Related Subject Headings

  • Sequence Deletion
  • Retinal Degeneration
  • Photoreceptor Cells
  • Pancreas, Exocrine
  • Nucleotide Motifs
  • Mice, Knockout
  • Mice
  • Kidney Diseases, Cystic
  • Kidney
  • Humans