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Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release.

Publication ,  Journal Article
Salinas, RY; Pearring, JN; Ding, J-D; Spencer, WJ; Hao, Y; Arshavsky, VY
Published in: J Cell Biol
May 1, 2017

The primary cilium is a highly conserved organelle housing specialized molecules responsible for receiving and processing extracellular signals. A recently discovered property shared across many cilia is the ability to release small vesicles called ectosomes, which are used for exchanging protein and genetic material among cells. In this study, we report a novel role for ciliary ectosomes in building the elaborate photoreceptor outer segment filled with hundreds of tightly packed "disc" membranes. We demonstrate that the photoreceptor cilium has an innate ability to release massive amounts of ectosomes. However, this process is suppressed by the disc-specific protein peripherin, which enables retained ectosomes to be morphed into discs. This new function of peripherin is performed independently from its well-established role in maintaining the high curvature of disc edges, and each function is fulfilled by a separate part of peripherin's molecule. Our findings explain how the outer segment structure evolved from the primary cilium to provide photoreceptor cells with vast membrane surfaces for efficient light capture.

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Published In

J Cell Biol

DOI

EISSN

1540-8140

Publication Date

May 1, 2017

Volume

216

Issue

5

Start / End Page

1489 / 1499

Location

United States

Related Subject Headings

  • Photoreceptor Cells
  • Peripherins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Developmental Biology
  • Cilia
  • Cell-Derived Microparticles
  • Animals
  • 32 Biomedical and clinical sciences
 

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Salinas, R. Y., Pearring, J. N., Ding, J.-D., Spencer, W. J., Hao, Y., & Arshavsky, V. Y. (2017). Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release. J Cell Biol, 216(5), 1489–1499. https://doi.org/10.1083/jcb.201608081
Salinas, Raquel Y., Jillian N. Pearring, Jin-Dong Ding, William J. Spencer, Ying Hao, and Vadim Y. Arshavsky. “Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release.J Cell Biol 216, no. 5 (May 1, 2017): 1489–99. https://doi.org/10.1083/jcb.201608081.
Salinas RY, Pearring JN, Ding J-D, Spencer WJ, Hao Y, Arshavsky VY. Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release. J Cell Biol. 2017 May 1;216(5):1489–99.
Salinas, Raquel Y., et al. “Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release.J Cell Biol, vol. 216, no. 5, May 2017, pp. 1489–99. Pubmed, doi:10.1083/jcb.201608081.
Salinas RY, Pearring JN, Ding J-D, Spencer WJ, Hao Y, Arshavsky VY. Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release. J Cell Biol. 2017 May 1;216(5):1489–1499.

Published In

J Cell Biol

DOI

EISSN

1540-8140

Publication Date

May 1, 2017

Volume

216

Issue

5

Start / End Page

1489 / 1499

Location

United States

Related Subject Headings

  • Photoreceptor Cells
  • Peripherins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Developmental Biology
  • Cilia
  • Cell-Derived Microparticles
  • Animals
  • 32 Biomedical and clinical sciences