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Screen and identification of proteins interacting with ADAM19 cytoplasmic tail.

Publication ,  Journal Article
Huang, L; Feng, L; Yang, L; Zhou, W; Zhao, S; Li, C
Published in: Mol Biol Rep
September 2002

ADAM family plays important roles in neurogenesis. The cytoplasmic tail of ADAM19 (ADAM19-CT) contains 193 residues. The presence of two putative SH3 ligand-binding sites suggests potential interactions with cytosolic proteins, which could be possibly linked to the functions of ADAM19. To address these issues, a yeast two-hybrid screen was performed in human fetal brain cDNA library to isolate proteins that interact with the cytoplasmic tail of ADAM19. Four proteins were obtained, ArgBP1, beta-cop, ubiquitin and a novel protein. GST-Pulldown assay has confirmed the interaction between AdAM19 and ArgBP1. By constructing series of deletion mutants of ADAM19-CT and ArgBP1 respectively, the interaction regions have been identified. They are the SH3 binding sites in ADAM19-CT and the P4 region in ArgBP1. And the interaction is specific. ArgBP1 does not bind to ADAM22, ADAM29 or ADAM9 (mouse). ArgBP1 may be the key protein, which accounts for the physiological function of ADAM19.

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Published In

Mol Biol Rep

DOI

ISSN

0301-4851

Publication Date

September 2002

Volume

29

Issue

3

Start / End Page

317 / 323

Location

Netherlands

Related Subject Headings

  • src Homology Domains
  • Ubiquitin
  • Two-Hybrid System Techniques
  • Proteins
  • Protein Binding
  • Muscle Proteins
  • Metalloproteases
  • Metalloendopeptidases
  • Membrane Proteins
  • Humans
 

Citation

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Huang, L., Feng, L., Yang, L., Zhou, W., Zhao, S., & Li, C. (2002). Screen and identification of proteins interacting with ADAM19 cytoplasmic tail. Mol Biol Rep, 29(3), 317–323. https://doi.org/10.1023/a:1020409217215
Huang, Li, Libing Feng, Limin Yang, Weiguo Zhou, Shouyuan Zhao, and Changben Li. “Screen and identification of proteins interacting with ADAM19 cytoplasmic tail.Mol Biol Rep 29, no. 3 (September 2002): 317–23. https://doi.org/10.1023/a:1020409217215.
Huang L, Feng L, Yang L, Zhou W, Zhao S, Li C. Screen and identification of proteins interacting with ADAM19 cytoplasmic tail. Mol Biol Rep. 2002 Sep;29(3):317–23.
Huang, Li, et al. “Screen and identification of proteins interacting with ADAM19 cytoplasmic tail.Mol Biol Rep, vol. 29, no. 3, Sept. 2002, pp. 317–23. Pubmed, doi:10.1023/a:1020409217215.
Huang L, Feng L, Yang L, Zhou W, Zhao S, Li C. Screen and identification of proteins interacting with ADAM19 cytoplasmic tail. Mol Biol Rep. 2002 Sep;29(3):317–323.
Journal cover image

Published In

Mol Biol Rep

DOI

ISSN

0301-4851

Publication Date

September 2002

Volume

29

Issue

3

Start / End Page

317 / 323

Location

Netherlands

Related Subject Headings

  • src Homology Domains
  • Ubiquitin
  • Two-Hybrid System Techniques
  • Proteins
  • Protein Binding
  • Muscle Proteins
  • Metalloproteases
  • Metalloendopeptidases
  • Membrane Proteins
  • Humans