Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry.
Publication
, Journal Article
Rudemiller, NP; Crowley, SD
Published in: Methods Mol Biol
2017
A variety of immune cell subsets contribute to the pathogenesis of hypertension and associated kidney damage following inappropriate activation of the renin-angiotensin system (RAS). These immune cell subsets often express common surface markers, which complicates their separation and characterization in vivo. Accordingly, flow cytometry has become an invaluable tool for parsing immune cell populations because this technique permits the simultaneous detection of up to 18 markers on a single cell. Below we describe a process by which one can determine the immune cell subsets in the kidney via flow cytometry.
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2017
Volume
1614
Start / End Page
87 / 98
Location
United States
Related Subject Headings
- T-Lymphocyte Subsets
- Renin-Angiotensin System
- Phenotype
- Mice
- Kidney
- Flow Cytometry
- Developmental Biology
- Cytokines
- Animals
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Rudemiller, N. P., & Crowley, S. D. (2017). Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry. Methods Mol Biol, 1614, 87–98. https://doi.org/10.1007/978-1-4939-7030-8_8
Rudemiller, Nathan P., and Steven D. Crowley. “Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry.” Methods Mol Biol 1614 (2017): 87–98. https://doi.org/10.1007/978-1-4939-7030-8_8.
Rudemiller NP, Crowley SD. Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry. Methods Mol Biol. 2017;1614:87–98.
Rudemiller, Nathan P., and Steven D. Crowley. “Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry.” Methods Mol Biol, vol. 1614, 2017, pp. 87–98. Pubmed, doi:10.1007/978-1-4939-7030-8_8.
Rudemiller NP, Crowley SD. Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry. Methods Mol Biol. 2017;1614:87–98.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2017
Volume
1614
Start / End Page
87 / 98
Location
United States
Related Subject Headings
- T-Lymphocyte Subsets
- Renin-Angiotensin System
- Phenotype
- Mice
- Kidney
- Flow Cytometry
- Developmental Biology
- Cytokines
- Animals
- 0601 Biochemistry and Cell Biology