Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation.

Publication ,  Journal Article
Tang, L; Navarro, LA; Chilkoti, A; Zauscher, S
Published in: Angewandte Chemie (International ed. in English)
June 2017

We present terminal deoxynucleotidyl transferase-catalyzed enzymatic polymerization (TcEP) for the template-free synthesis of high-molecular-weight, single-stranded DNA (ssDNA) and demonstrate that it proceeds by a living chain-growth polycondensation mechanism. We show that the molecular weight of the reaction products is nearly monodisperse, and can be manipulated by the feed ratio of nucleotide (monomer) to oligonucleotide (initiator), as typically observed for living polymerization reactions. Understanding the synthesis mechanism and the reaction kinetics enables the rational, template-free synthesis of ssDNA that can be used for a range of biomedical and nanotechnology applications.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

June 2017

Volume

56

Issue

24

Start / End Page

6778 / 6782

Related Subject Headings

  • Polynucleotides
  • Polymerization
  • Organic Chemistry
  • Nanotechnology
  • Molecular Weight
  • Kinetics
  • DNA, Single-Stranded
  • DNA Nucleotidylexotransferase
  • Catalysis
  • 34 Chemical sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tang, L., Navarro, L. A., Chilkoti, A., & Zauscher, S. (2017). High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation. Angewandte Chemie (International Ed. in English), 56(24), 6778–6782. https://doi.org/10.1002/anie.201700991
Tang, Lei, Luis A. Navarro, Ashutosh Chilkoti, and Stefan Zauscher. “High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation.Angewandte Chemie (International Ed. in English) 56, no. 24 (June 2017): 6778–82. https://doi.org/10.1002/anie.201700991.
Tang L, Navarro LA, Chilkoti A, Zauscher S. High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation. Angewandte Chemie (International ed in English). 2017 Jun;56(24):6778–82.
Tang, Lei, et al. “High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation.Angewandte Chemie (International Ed. in English), vol. 56, no. 24, June 2017, pp. 6778–82. Epmc, doi:10.1002/anie.201700991.
Tang L, Navarro LA, Chilkoti A, Zauscher S. High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation. Angewandte Chemie (International ed in English). 2017 Jun;56(24):6778–6782.
Journal cover image

Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

June 2017

Volume

56

Issue

24

Start / End Page

6778 / 6782

Related Subject Headings

  • Polynucleotides
  • Polymerization
  • Organic Chemistry
  • Nanotechnology
  • Molecular Weight
  • Kinetics
  • DNA, Single-Stranded
  • DNA Nucleotidylexotransferase
  • Catalysis
  • 34 Chemical sciences