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ZipA and FtsA* stabilize FtsZ-GDP miniring structures.

Publication ,  Journal Article
Chen, Y; Huang, H; Osawa, M; Erickson, HP
Published in: Sci Rep
June 16, 2017

The cytokinetic division ring of Escherichia coli comprises filaments of FtsZ tethered to the membrane by FtsA and ZipA. Previous results suggested that ZipA is a Z-ring stabilizer, since in vitro experiments it is shown that ZipA enhanced FtsZ assembly and caused the filaments to bundles. However, this function of ZipA has been challenged by recent studies. First, ZipA-induced FtsZ bundling was not significant at pH greater than 7. Second, some FtsA mutants, such as FtsA* were able to bypass the need of ZipA. We reinvestigated the interaction of FtsZ with ZipA in vitro. We found that ZipA not only stabilized and bundled straight filaments of FtsZ-GTP, but also stabilized the highly curved filaments and miniring structures formed by FtsZ-GDP. FtsA* had a similar stabilization of FtsZ-GDP minirings. Our results suggest that ZipA and FtsA* may contribute to constriction by stabilizing this miniring conformation.

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

June 16, 2017

Volume

7

Issue

1

Start / End Page

3650

Location

England

Related Subject Headings

  • Structure-Activity Relationship
  • Protein Stability
  • Protein Binding
  • Guanosine Triphosphate
  • Guanosine Diphosphate
  • Escherichia coli Proteins
  • Cytoskeleton
  • Cytoskeletal Proteins
  • Cell Cycle Proteins
  • Carrier Proteins
 

Citation

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Chen, Y., Huang, H., Osawa, M., & Erickson, H. P. (2017). ZipA and FtsA* stabilize FtsZ-GDP miniring structures. Sci Rep, 7(1), 3650. https://doi.org/10.1038/s41598-017-03983-4
Chen, Yaodong, Haiyan Huang, Masaki Osawa, and Harold P. Erickson. “ZipA and FtsA* stabilize FtsZ-GDP miniring structures.Sci Rep 7, no. 1 (June 16, 2017): 3650. https://doi.org/10.1038/s41598-017-03983-4.
Chen Y, Huang H, Osawa M, Erickson HP. ZipA and FtsA* stabilize FtsZ-GDP miniring structures. Sci Rep. 2017 Jun 16;7(1):3650.
Chen, Yaodong, et al. “ZipA and FtsA* stabilize FtsZ-GDP miniring structures.Sci Rep, vol. 7, no. 1, June 2017, p. 3650. Pubmed, doi:10.1038/s41598-017-03983-4.
Chen Y, Huang H, Osawa M, Erickson HP. ZipA and FtsA* stabilize FtsZ-GDP miniring structures. Sci Rep. 2017 Jun 16;7(1):3650.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

June 16, 2017

Volume

7

Issue

1

Start / End Page

3650

Location

England

Related Subject Headings

  • Structure-Activity Relationship
  • Protein Stability
  • Protein Binding
  • Guanosine Triphosphate
  • Guanosine Diphosphate
  • Escherichia coli Proteins
  • Cytoskeleton
  • Cytoskeletal Proteins
  • Cell Cycle Proteins
  • Carrier Proteins