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Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST).

Publication ,  Journal Article
Wu, Q; Fenton, BA; Wojtaszek, JL; Zhou, P
Published in: Chem Commun (Camb)
July 27, 2017

CEST-NMR spectroscopy is a powerful tool for probing the conformational dynamics of macromolecules. We present a HNdec-CEST experiment that simplifies the relaxation matrix, reduces fitting parameters, and enhances signal resolution. Importantly, fitting of HNdec-CEST profiles enables robust extraction of exchange rates as well as excited-state chemical shifts and populations.

Duke Scholars

Published In

Chem Commun (Camb)

DOI

EISSN

1364-548X

Publication Date

July 27, 2017

Volume

53

Issue

61

Start / End Page

8541 / 8544

Location

England

Related Subject Headings

  • Organic Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

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Wu, Q., Fenton, B. A., Wojtaszek, J. L., & Zhou, P. (2017). Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST). Chem Commun (Camb), 53(61), 8541–8544. https://doi.org/10.1039/c7cc05021f
Wu, Qinglin, Benjamin A. Fenton, Jessica L. Wojtaszek, and Pei Zhou. “Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST).Chem Commun (Camb) 53, no. 61 (July 27, 2017): 8541–44. https://doi.org/10.1039/c7cc05021f.
Wu, Qinglin, et al. “Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST).Chem Commun (Camb), vol. 53, no. 61, July 2017, pp. 8541–44. Pubmed, doi:10.1039/c7cc05021f.
Journal cover image

Published In

Chem Commun (Camb)

DOI

EISSN

1364-548X

Publication Date

July 27, 2017

Volume

53

Issue

61

Start / End Page

8541 / 8544

Location

England

Related Subject Headings

  • Organic Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 03 Chemical Sciences