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Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia.

Publication ,  Journal Article
Chien, JS; Mohammed, M; Eldik, H; Ibrahim, MM; Martinez, J; Nichols, SP; Wisniewski, N; Klitzman, B
Published in: Sci Rep
August 15, 2017

Novel injectable biosensors were used to measure interstitial oxygenation before, during, and after transient ischemia. It is well known that reactive hyperemia occurs following a period of ischemia. However, increased blood flow does not necessarily mean increased oxygen tension in the tissue. Therefore, the purpose of this study was to test the hypothesis that tissue reactive hyperoxia occurs following release of hind-limb tourniquet occlusions. Rats were injected with bilateral hind-limb biosensors and were simultaneously subjected to a unilateral femoral vessel ligation. After approximately one and three months, the rats underwent a series of oxygenation challenges, including transient hind-limb tourniquet occlusion. Along with the biosensors, near infrared spectroscopy was used to measure percent oxyhemoglobin in capillaries and laser Doppler flowmetry was used to measure blood flow. Post-occlusion reactive hyperemia was observed. It was accompanied by tissue reactive hyperoxia, affirming that the post-occlusion oxygen supply must have exceeded the expected increased oxygen consumption. The measurement of the physiologic phenomenon of reactive hyperoxia could prove clinically beneficial for both diagnosis and optimizing therapy.

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

August 15, 2017

Volume

7

Issue

1

Start / End Page

8255

Location

England

Related Subject Headings

  • Time Factors
  • Regional Blood Flow
  • Rats
  • Oxygen Consumption
  • Oxygen
  • Luminescent Agents
  • Lower Extremity
  • Laser-Doppler Flowmetry
  • Ischemia
  • Hyperoxia
 

Citation

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Chien, J. S., Mohammed, M., Eldik, H., Ibrahim, M. M., Martinez, J., Nichols, S. P., … Klitzman, B. (2017). Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia. Sci Rep, 7(1), 8255. https://doi.org/10.1038/s41598-017-08490-0
Chien, Jennifer S., Mahmoud Mohammed, Hysem Eldik, Mohamed M. Ibrahim, Jeremy Martinez, Scott P. Nichols, Natalie Wisniewski, and Bruce Klitzman. “Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia.Sci Rep 7, no. 1 (August 15, 2017): 8255. https://doi.org/10.1038/s41598-017-08490-0.
Chien JS, Mohammed M, Eldik H, Ibrahim MM, Martinez J, Nichols SP, et al. Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia. Sci Rep. 2017 Aug 15;7(1):8255.
Chien, Jennifer S., et al. “Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia.Sci Rep, vol. 7, no. 1, Aug. 2017, p. 8255. Pubmed, doi:10.1038/s41598-017-08490-0.
Chien JS, Mohammed M, Eldik H, Ibrahim MM, Martinez J, Nichols SP, Wisniewski N, Klitzman B. Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia. Sci Rep. 2017 Aug 15;7(1):8255.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

August 15, 2017

Volume

7

Issue

1

Start / End Page

8255

Location

England

Related Subject Headings

  • Time Factors
  • Regional Blood Flow
  • Rats
  • Oxygen Consumption
  • Oxygen
  • Luminescent Agents
  • Lower Extremity
  • Laser-Doppler Flowmetry
  • Ischemia
  • Hyperoxia