Skip to main content

Extracellular-matrix-based and Arg-Gly-Asp-modified photopolymerizing hydrogels for cartilage tissue engineering.

Publication ,  Journal Article
Kim, HD; Heo, J; Hwang, Y; Kwak, S-Y; Park, OK; Kim, H; Varghese, S; Hwang, NS
Published in: Tissue Eng Part A
February 2015

Articular cartilage damage is a persistent and increasing problem with the aging population. Strategies to achieve complete repair or functional restoration remain a challenge. Photopolymerizing-based hydrogels have long received an attention in the cartilage tissue engineering, due to their unique bioactivities, flexible method of synthesis, range of constituents, and desirable physical characteristics. In the present study, we have introduced unique bioactivity within the photopolymerizing-based hydrogels by copolymerizing polyethylene glycol (PEG) macromers with methacrylated extracellular matrix (ECM) molecules (hyaluronic acid and chondroitin sulfate [CS]) and integrin binding peptides (RGD peptide). Results indicate that cellular morphology, as observed by the actin cytoskeleton structures, was strongly dependent on the type of ECM component as well as the presence of integrin binding moieties. Further, CS-based hydrogel with integrin binding RGD moieties increased the lubricin (or known as superficial zone protein [SZP]) gene expression of the encapsulated chondrocytes. Additionally, CS-based hydrogel displayed cell-responsive degradation and resulted in increased DNA, GAG, and collagen accumulation compared with other hydrogels. This study demonstrates that integrin-mediated interactions within CS microenvironment provide an optimal hydrogel scaffold for cartilage tissue engineering application.

Duke Scholars

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

February 2015

Volume

21

Issue

3-4

Start / End Page

757 / 766

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Photochemistry
  • Oligopeptides
  • Materials Testing
  • Light
  • Hydrogels
  • Extracellular Matrix
  • Equipment Failure Analysis
  • Equipment Design
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kim, H. D., Heo, J., Hwang, Y., Kwak, S.-Y., Park, O. K., Kim, H., … Hwang, N. S. (2015). Extracellular-matrix-based and Arg-Gly-Asp-modified photopolymerizing hydrogels for cartilage tissue engineering. Tissue Eng Part A, 21(3–4), 757–766. https://doi.org/10.1089/ten.TEA.2014.0233
Kim, Hwan D., Jiseung Heo, Yongsung Hwang, Seon-Yeong Kwak, Ok Kyu Park, Hyunbum Kim, Shyni Varghese, and Nathaniel S. Hwang. “Extracellular-matrix-based and Arg-Gly-Asp-modified photopolymerizing hydrogels for cartilage tissue engineering.Tissue Eng Part A 21, no. 3–4 (February 2015): 757–66. https://doi.org/10.1089/ten.TEA.2014.0233.
Kim HD, Heo J, Hwang Y, Kwak S-Y, Park OK, Kim H, et al. Extracellular-matrix-based and Arg-Gly-Asp-modified photopolymerizing hydrogels for cartilage tissue engineering. Tissue Eng Part A. 2015 Feb;21(3–4):757–66.
Kim, Hwan D., et al. “Extracellular-matrix-based and Arg-Gly-Asp-modified photopolymerizing hydrogels for cartilage tissue engineering.Tissue Eng Part A, vol. 21, no. 3–4, Feb. 2015, pp. 757–66. Pubmed, doi:10.1089/ten.TEA.2014.0233.
Kim HD, Heo J, Hwang Y, Kwak S-Y, Park OK, Kim H, Varghese S, Hwang NS. Extracellular-matrix-based and Arg-Gly-Asp-modified photopolymerizing hydrogels for cartilage tissue engineering. Tissue Eng Part A. 2015 Feb;21(3–4):757–766.

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

February 2015

Volume

21

Issue

3-4

Start / End Page

757 / 766

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Photochemistry
  • Oligopeptides
  • Materials Testing
  • Light
  • Hydrogels
  • Extracellular Matrix
  • Equipment Failure Analysis
  • Equipment Design