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In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells.

Publication ,  Journal Article
Hwang, NS; Varghese, S; Lee, HJ; Zhang, Z; Ye, Z; Bae, J; Cheng, L; Elisseeff, J
Published in: Proc Natl Acad Sci U S A
December 30, 2008

Development of clinically relevant regenerative medicine therapies using human embryonic stem cells (hESCs) requires production of a simple and readily expandable cell population that can be directed to form functional 3D tissue in an in vivo environment. We describe an efficient derivation method and characterization of mesenchymal stem cells (MSCs) from hESCs (hESCd-MSCs) that have multilineage differentiation potential and are capable of producing fat, cartilage, and bone in vitro. Furthermore, we highlight their in vivo survival and commitment to the chondrogenic lineage in a microenvironment comprising chondrocyte-secreted morphogenetic factors and hydrogels. Normal cartilage architecture was established in rat osteochondral defects after treatment with chondrogenically-committed hESCd-MSCs. In view of the limited available cell sources for tissue engineering applications, these embryonic-derived cells show significant potential in musculoskeletal tissue regeneration applications.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

December 30, 2008

Volume

105

Issue

52

Start / End Page

20641 / 20646

Location

United States

Related Subject Headings

  • Regeneration
  • Rats
  • Mice, Nude
  • Mice
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Humans
  • Embryonic Stem Cells
  • Chondrocytes
  • Cell Survival
 

Citation

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Hwang, N. S., Varghese, S., Lee, H. J., Zhang, Z., Ye, Z., Bae, J., … Elisseeff, J. (2008). In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells. Proc Natl Acad Sci U S A, 105(52), 20641–20646. https://doi.org/10.1073/pnas.0809680106
Hwang, Nathaniel S., Shyni Varghese, H Janice Lee, Zijun Zhang, Zhaohui Ye, Jongwoo Bae, Linzhao Cheng, and Jennifer Elisseeff. “In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells.Proc Natl Acad Sci U S A 105, no. 52 (December 30, 2008): 20641–46. https://doi.org/10.1073/pnas.0809680106.
Hwang NS, Varghese S, Lee HJ, Zhang Z, Ye Z, Bae J, et al. In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells. Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20641–6.
Hwang, Nathaniel S., et al. “In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells.Proc Natl Acad Sci U S A, vol. 105, no. 52, Dec. 2008, pp. 20641–46. Pubmed, doi:10.1073/pnas.0809680106.
Hwang NS, Varghese S, Lee HJ, Zhang Z, Ye Z, Bae J, Cheng L, Elisseeff J. In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells. Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20641–20646.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

December 30, 2008

Volume

105

Issue

52

Start / End Page

20641 / 20646

Location

United States

Related Subject Headings

  • Regeneration
  • Rats
  • Mice, Nude
  • Mice
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Humans
  • Embryonic Stem Cells
  • Chondrocytes
  • Cell Survival