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Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels.

Publication ,  Journal Article
Hwang, NS; Varghese, S; Zhang, Z; Elisseeff, J
Published in: Tissue Eng
September 2006

Human embryonic stem cells (hESCs) have the potential to self-renew and generate multiple cell types, producing critical building blocks for tissue engineering and regenerative medicine applications. Here, we describe the efficient derivation and chondrogenic differentiation of mesenchymal-like cells from hESCs. These cells exhibit mesenchymal stem cell (MSC) surface markers, including CD29, CD44, CD105, and platelet-derived growth factor receptor-alpha. Under appropriate growth conditions, the hESC-derived cells proliferated without phenotypic changes and maintained MSC surface markers. The chondrogenic capacity of the cells was studied in pellet culture and after encapsulation in poly(ethylene glycol)-diacrylate (PEGDA) hydrogels with exogenous extracellular proteins or arginineglycine- aspartate (RGD)-modified PEGDA hydrogels. The hESC-derived cells exhibited growth factor- dependent matrix production in pellet culture but did not produce tissue characteristic of cartilage morphology. In PEGDA hydrogels containing exogenous hyaluronic acid or type I collagen, no significant cell growth or matrix production was observed. In contrast, when these cells were encapsulated in RGDmodified poly(ethylene glycol)hydrogels, neocartilage with basophilic extracellular matrix deposition was observed within 3 weeks of culture, producing cartilage-specific gene up-regulation and extracellular matrix production. Our results indicate that precursor cells characteristic of a MSC population can be cultured from differentiating hESCs through embryoid bodies, thus holding great promise for a potentially unlimited source of cells for cartilage tissue engineering.

Duke Scholars

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Published In

Tissue Eng

DOI

ISSN

1076-3279

Publication Date

September 2006

Volume

12

Issue

9

Start / End Page

2695 / 2706

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Polyethylene Glycols
  • Oligopeptides
  • Mesenchymal Stem Cells
  • Hydrogels
  • Humans
  • Extracellular Matrix
  • Embryo, Mammalian
  • Chondrogenesis
  • Cells, Immobilized
 

Citation

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Hwang, N. S., Varghese, S., Zhang, Z., & Elisseeff, J. (2006). Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels. Tissue Eng, 12(9), 2695–2706. https://doi.org/10.1089/ten.2006.12.2695
Hwang, Nathaniel S., Shyni Varghese, Zijun Zhang, and Jennifer Elisseeff. “Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels.Tissue Eng 12, no. 9 (September 2006): 2695–2706. https://doi.org/10.1089/ten.2006.12.2695.
Hwang NS, Varghese S, Zhang Z, Elisseeff J. Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels. Tissue Eng. 2006 Sep;12(9):2695–706.
Hwang, Nathaniel S., et al. “Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels.Tissue Eng, vol. 12, no. 9, Sept. 2006, pp. 2695–706. Pubmed, doi:10.1089/ten.2006.12.2695.
Hwang NS, Varghese S, Zhang Z, Elisseeff J. Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels. Tissue Eng. 2006 Sep;12(9):2695–2706.

Published In

Tissue Eng

DOI

ISSN

1076-3279

Publication Date

September 2006

Volume

12

Issue

9

Start / End Page

2695 / 2706

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Polyethylene Glycols
  • Oligopeptides
  • Mesenchymal Stem Cells
  • Hydrogels
  • Humans
  • Extracellular Matrix
  • Embryo, Mammalian
  • Chondrogenesis
  • Cells, Immobilized