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Interconnected macroporous poly(ethylene glycol) cryogels as a cell scaffold for cartilage tissue engineering.

Publication ,  Journal Article
Hwang, Y; Sangaj, N; Varghese, S
Published in: Tissue Eng Part A
October 2010

Macroporous networks of poly(ethylene glycol) (PEG) with interconnected pores can be created by cryogelation techniques. In this study, we describe the potential application of such PEG cryogels as scaffolds for cartilage tissue engineering. Three-dimensional macroporous cryogels were evaluated for chondrocyte growth and production of cartilage-specific extracellular matrix (ECM). Seeded primary bovine chondrocytes showed homogeneous distribution throughout the cryogels. DNA content suggests continuous cell proliferation over 4 weeks of in vitro culture. Analysis of the composition of cell-secreted ECM showed a culture-time-dependent increase in the amount of glycosaminoglycan and collagen. The production of ECM by chondrocytes was confirmed using scanning electron microscopy analysis. Further histological and immunohistological analysis of the cell-laden scaffold confirmed the presence of accumulated cartilage-specific ECM within the scaffold. The interconnected macroporous network promoted diffusion of cell-secreted matrix within the cryogels. Our results indicated that interconnected macroporous PEG cryogels successfully supported attachment, viability, proliferation, and biosynthetic activity of seeded chondrocytes.

Duke Scholars

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

October 2010

Volume

16

Issue

10

Start / End Page

3033 / 3041

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Polyethylene Glycols
  • Microscopy, Electron, Scanning
  • Immunohistochemistry
  • Hydrogels
  • Extracellular Matrix
  • Cryogels
  • Chondrocytes
  • Cells, Cultured
 

Citation

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ICMJE
MLA
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Hwang, Y., Sangaj, N., & Varghese, S. (2010). Interconnected macroporous poly(ethylene glycol) cryogels as a cell scaffold for cartilage tissue engineering. Tissue Eng Part A, 16(10), 3033–3041. https://doi.org/10.1089/ten.TEA.2010.0045
Hwang, Yongsung, Nivedita Sangaj, and Shyni Varghese. “Interconnected macroporous poly(ethylene glycol) cryogels as a cell scaffold for cartilage tissue engineering.Tissue Eng Part A 16, no. 10 (October 2010): 3033–41. https://doi.org/10.1089/ten.TEA.2010.0045.
Hwang Y, Sangaj N, Varghese S. Interconnected macroporous poly(ethylene glycol) cryogels as a cell scaffold for cartilage tissue engineering. Tissue Eng Part A. 2010 Oct;16(10):3033–41.
Hwang, Yongsung, et al. “Interconnected macroporous poly(ethylene glycol) cryogels as a cell scaffold for cartilage tissue engineering.Tissue Eng Part A, vol. 16, no. 10, Oct. 2010, pp. 3033–41. Pubmed, doi:10.1089/ten.TEA.2010.0045.
Hwang Y, Sangaj N, Varghese S. Interconnected macroporous poly(ethylene glycol) cryogels as a cell scaffold for cartilage tissue engineering. Tissue Eng Part A. 2010 Oct;16(10):3033–3041.

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

October 2010

Volume

16

Issue

10

Start / End Page

3033 / 3041

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Polyethylene Glycols
  • Microscopy, Electron, Scanning
  • Immunohistochemistry
  • Hydrogels
  • Extracellular Matrix
  • Cryogels
  • Chondrocytes
  • Cells, Cultured