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Screening of primary gp120 immunogens to formulate the next generation polyvalent DNA prime-protein boost HIV-1 vaccines.

Publication ,  Journal Article
Wang, S; Chou, T-H; Hackett, A; Efros, V; Wang, Y; Han, D; Wallace, A; Chen, Y; Hu, G; Liu, S; Clapham, P; Arthos, J; Montefiori, D; Lu, S
Published in: Hum Vaccin Immunother
December 2, 2017

Our previous preclinical studies and a Phase I clinical trial DP6-001 have indicated that a polyvalent Env formulation was able to elicit broadly reactive antibody responses including low titer neutralizing antibody responses against viral isolates of subtypes A, B, C and AE. In the current report, a panel of 62 gp120 immunogens were screened in a rabbit model to identify gp120 immunogens that can elicit improved binding and neutralizing antibody responses and some of them can be included in the next polyvalent formulation. Only about 19% of gp120 immunogens in this panel were able to elicit neutralizing antibodies against greater than 50% of the viruses included in a high throughput PhenoSense neutralization assay when these immuongens were tested as a DNA prime followed by a fixed 5-valent gp120 protein vaccine boost. The new polyvalent formulation, using five gp120 immunogens selected from this subgroup, elicited improved quality of antibody responses in rabbits than the previous DP6-001 formulation. More significantly, this new polyvalent formulation elicited higher antibody responses against a panel of gp70V1/V2 antigens expressing V1/V2 sequences from diverse subtypes. Bioinformatics analysis supports the design of a 4-valent or 5-valent formulation using gp120 immunogens from this screening study to achieve a broad coverage against 16 HIV-1 subtypes.

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Published In

Hum Vaccin Immunother

DOI

EISSN

2164-554X

Publication Date

December 2, 2017

Volume

13

Issue

12

Start / End Page

2996 / 3009

Location

United States

Related Subject Headings

  • Vaccines, Subunit
  • Vaccines, DNA
  • Rabbits
  • Neutralization Tests
  • Immunization Schedule
  • HIV Envelope Protein gp120
  • HIV Antibodies
  • Drug Evaluation, Preclinical
  • Antibodies, Neutralizing
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
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Wang, S., Chou, T.-H., Hackett, A., Efros, V., Wang, Y., Han, D., … Lu, S. (2017). Screening of primary gp120 immunogens to formulate the next generation polyvalent DNA prime-protein boost HIV-1 vaccines. Hum Vaccin Immunother, 13(12), 2996–3009. https://doi.org/10.1080/21645515.2017.1380137
Wang, Shixia, Te-Hui Chou, Anthony Hackett, Veronica Efros, Yan Wang, Dong Han, Aaron Wallace, et al. “Screening of primary gp120 immunogens to formulate the next generation polyvalent DNA prime-protein boost HIV-1 vaccines.Hum Vaccin Immunother 13, no. 12 (December 2, 2017): 2996–3009. https://doi.org/10.1080/21645515.2017.1380137.
Wang S, Chou T-H, Hackett A, Efros V, Wang Y, Han D, et al. Screening of primary gp120 immunogens to formulate the next generation polyvalent DNA prime-protein boost HIV-1 vaccines. Hum Vaccin Immunother. 2017 Dec 2;13(12):2996–3009.
Wang, Shixia, et al. “Screening of primary gp120 immunogens to formulate the next generation polyvalent DNA prime-protein boost HIV-1 vaccines.Hum Vaccin Immunother, vol. 13, no. 12, Dec. 2017, pp. 2996–3009. Pubmed, doi:10.1080/21645515.2017.1380137.
Wang S, Chou T-H, Hackett A, Efros V, Wang Y, Han D, Wallace A, Chen Y, Hu G, Liu S, Clapham P, Arthos J, Montefiori D, Lu S. Screening of primary gp120 immunogens to formulate the next generation polyvalent DNA prime-protein boost HIV-1 vaccines. Hum Vaccin Immunother. 2017 Dec 2;13(12):2996–3009.

Published In

Hum Vaccin Immunother

DOI

EISSN

2164-554X

Publication Date

December 2, 2017

Volume

13

Issue

12

Start / End Page

2996 / 3009

Location

United States

Related Subject Headings

  • Vaccines, Subunit
  • Vaccines, DNA
  • Rabbits
  • Neutralization Tests
  • Immunization Schedule
  • HIV Envelope Protein gp120
  • HIV Antibodies
  • Drug Evaluation, Preclinical
  • Antibodies, Neutralizing
  • Animals