Skip to main content
Journal cover image

Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms.

Publication ,  Journal Article
Shwab, EK; Juvvadi, PR; Waitt, G; Soderblom, EJ; Moseley, MA; Nicely, NI; Steinbach, WJ
Published in: FEBS Lett
November 2017

Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subunit PkaR, distinct from those previously identified in mammals and yeasts, and demonstrated the importance of two phosphorylation clusters for hyphal growth and cell wall-stress response. We also identified key differences in the regulation of PKA subcellular localization in A. fumigatus compared with other species. This is the first analysis of the phosphoregulation of a PKA regulatory subunit in a filamentous fungus and uncovers critical mechanistic differences between PKA regulation in filamentous fungi compared with mammals and yeast species, suggesting divergent targeting opportunities.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

FEBS Lett

DOI

EISSN

1873-3468

Publication Date

November 2017

Volume

591

Issue

22

Start / End Page

3730 / 3744

Location

England

Related Subject Headings

  • Tandem Mass Spectrometry
  • Phosphorylation
  • Mutation
  • Models, Molecular
  • Gene Expression Regulation, Fungal
  • Fungal Proteins
  • Cyclic AMP-Dependent Protein Kinases
  • Chromatography, Liquid
  • Cell Wall
  • Biochemistry & Molecular Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shwab, E. K., Juvvadi, P. R., Waitt, G., Soderblom, E. J., Moseley, M. A., Nicely, N. I., & Steinbach, W. J. (2017). Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms. FEBS Lett, 591(22), 3730–3744. https://doi.org/10.1002/1873-3468.12886
Shwab, Elliot K., Praveen R. Juvvadi, Greg Waitt, Erik J. Soderblom, Martin A. Moseley, Nathan I. Nicely, and William J. Steinbach. “Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms.FEBS Lett 591, no. 22 (November 2017): 3730–44. https://doi.org/10.1002/1873-3468.12886.
Shwab EK, Juvvadi PR, Waitt G, Soderblom EJ, Moseley MA, Nicely NI, et al. Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms. FEBS Lett. 2017 Nov;591(22):3730–44.
Shwab, Elliot K., et al. “Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms.FEBS Lett, vol. 591, no. 22, Nov. 2017, pp. 3730–44. Pubmed, doi:10.1002/1873-3468.12886.
Shwab EK, Juvvadi PR, Waitt G, Soderblom EJ, Moseley MA, Nicely NI, Steinbach WJ. Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms. FEBS Lett. 2017 Nov;591(22):3730–3744.
Journal cover image

Published In

FEBS Lett

DOI

EISSN

1873-3468

Publication Date

November 2017

Volume

591

Issue

22

Start / End Page

3730 / 3744

Location

England

Related Subject Headings

  • Tandem Mass Spectrometry
  • Phosphorylation
  • Mutation
  • Models, Molecular
  • Gene Expression Regulation, Fungal
  • Fungal Proteins
  • Cyclic AMP-Dependent Protein Kinases
  • Chromatography, Liquid
  • Cell Wall
  • Biochemistry & Molecular Biology