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A mouse model of adult-onset anaemia due to erythropoietin deficiency.

Publication ,  Journal Article
Yamazaki, S; Souma, T; Hirano, I; Pan, X; Minegishi, N; Suzuki, N; Yamamoto, M
Published in: Nat Commun
2013

Erythropoietin regulates erythropoiesis in a hypoxia-inducible manner. Here we generate inherited super-anaemic mice (ISAM) as a mouse model of adult-onset anaemia caused by erythropoietin deficiency. ISAM express erythropoietin in the liver but lack erythropoietin production in the kidney. Around weaning age, when the major erythropoietin-producing organ switches from the liver to the kidney, ISAM develop anaemia due to erythropoietin deficiency, which is curable by administration of recombinant erythropoietin. In ISAM severe chronic anaemia enhances transgenic green fluorescent protein and Cre expression driven by the complete erythropoietin-gene regulatory regions, which facilitates efficient labelling of renal erythropoietin-producing cells. We show that the majority of cortical and outer medullary fibroblasts have the innate potential to produce erythropoietin, and also reveal a new set of erythropoietin target genes. ISAM are a useful tool for the evaluation of erythropoiesis-stimulating agents and to trace the dynamics of erythropoietin-producing cells.

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Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

2013

Volume

4

Start / End Page

1950

Location

England

Related Subject Headings

  • Transgenes
  • Staining and Labeling
  • RNA, Messenger
  • Mice, Knockout
  • Mice
  • Liver
  • Kidney
  • Integrases
  • Humans
  • Hematopoiesis
 

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Yamazaki, S., Souma, T., Hirano, I., Pan, X., Minegishi, N., Suzuki, N., & Yamamoto, M. (2013). A mouse model of adult-onset anaemia due to erythropoietin deficiency. Nat Commun, 4, 1950. https://doi.org/10.1038/ncomms2950
Yamazaki, Shun, Tomokazu Souma, Ikuo Hirano, Xiaoqing Pan, Naoko Minegishi, Norio Suzuki, and Masayuki Yamamoto. “A mouse model of adult-onset anaemia due to erythropoietin deficiency.Nat Commun 4 (2013): 1950. https://doi.org/10.1038/ncomms2950.
Yamazaki S, Souma T, Hirano I, Pan X, Minegishi N, Suzuki N, et al. A mouse model of adult-onset anaemia due to erythropoietin deficiency. Nat Commun. 2013;4:1950.
Yamazaki, Shun, et al. “A mouse model of adult-onset anaemia due to erythropoietin deficiency.Nat Commun, vol. 4, 2013, p. 1950. Pubmed, doi:10.1038/ncomms2950.
Yamazaki S, Souma T, Hirano I, Pan X, Minegishi N, Suzuki N, Yamamoto M. A mouse model of adult-onset anaemia due to erythropoietin deficiency. Nat Commun. 2013;4:1950.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

2013

Volume

4

Start / End Page

1950

Location

England

Related Subject Headings

  • Transgenes
  • Staining and Labeling
  • RNA, Messenger
  • Mice, Knockout
  • Mice
  • Liver
  • Kidney
  • Integrases
  • Humans
  • Hematopoiesis