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Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain.

Publication ,  Journal Article
Naegeli, KM; Hastie, E; Garde, A; Wang, Z; Keeley, DP; Gordon, KL; Pani, AM; Kelley, LC; Morrissey, MA; Chi, Q; Goldstein, B; Sherwood, DR
Published in: Developmental cell
November 2017

Invasive cells use small invadopodia to breach basement membrane (BM), a dense matrix that encases tissues. Following the breach, a large protrusion forms to clear a path for tissue entry by poorly understood mechanisms. Using RNAi screening for defects in Caenorhabditis elegans anchor cell (AC) invasion, we found that UNC-6(netrin)/UNC-40(DCC) signaling at the BM breach site directs exocytosis of lysosomes using the exocyst and SNARE SNAP-29 to form a large protrusion that invades vulval tissue. Live-cell imaging revealed that the protrusion is enriched in the matrix metalloprotease ZMP-1 and transiently expands AC volume by more than 20%, displacing surrounding BM and vulval epithelium. Photobleaching and genetic perturbations showed that the BM receptor dystroglycan forms a membrane diffusion barrier at the neck of the protrusion, which enables protrusion growth. Together these studies define a netrin-dependent pathway that builds an invasive protrusion, an isolated lysosome-derived membrane structure specialized to breach tissue barriers.

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Published In

Developmental cell

DOI

EISSN

1878-1551

ISSN

1534-5807

Publication Date

November 2017

Volume

43

Issue

4

Start / End Page

403 / 417.e10

Related Subject Headings

  • Nerve Tissue Proteins
  • Lysosomes
  • Gene Expression Regulation, Developmental
  • Exocytosis
  • Developmental Biology
  • Cell Movement
  • Cell Membrane
  • Caenorhabditis elegans Proteins
  • Caenorhabditis elegans
  • Basement Membrane
 

Citation

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MLA
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Naegeli, K. M., Hastie, E., Garde, A., Wang, Z., Keeley, D. P., Gordon, K. L., … Sherwood, D. R. (2017). Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain. Developmental Cell, 43(4), 403-417.e10. https://doi.org/10.1016/j.devcel.2017.10.024
Naegeli, Kaleb M., Eric Hastie, Aastha Garde, Zheng Wang, Daniel P. Keeley, Kacy L. Gordon, Ariel M. Pani, et al. “Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain.Developmental Cell 43, no. 4 (November 2017): 403-417.e10. https://doi.org/10.1016/j.devcel.2017.10.024.
Naegeli KM, Hastie E, Garde A, Wang Z, Keeley DP, Gordon KL, et al. Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain. Developmental cell. 2017 Nov;43(4):403-417.e10.
Naegeli, Kaleb M., et al. “Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain.Developmental Cell, vol. 43, no. 4, Nov. 2017, pp. 403-417.e10. Epmc, doi:10.1016/j.devcel.2017.10.024.
Naegeli KM, Hastie E, Garde A, Wang Z, Keeley DP, Gordon KL, Pani AM, Kelley LC, Morrissey MA, Chi Q, Goldstein B, Sherwood DR. Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain. Developmental cell. 2017 Nov;43(4):403-417.e10.
Journal cover image

Published In

Developmental cell

DOI

EISSN

1878-1551

ISSN

1534-5807

Publication Date

November 2017

Volume

43

Issue

4

Start / End Page

403 / 417.e10

Related Subject Headings

  • Nerve Tissue Proteins
  • Lysosomes
  • Gene Expression Regulation, Developmental
  • Exocytosis
  • Developmental Biology
  • Cell Movement
  • Cell Membrane
  • Caenorhabditis elegans Proteins
  • Caenorhabditis elegans
  • Basement Membrane