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Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides.

Publication ,  Journal Article
Costa, SA; Simon, JR; Amiram, M; Tang, L; Zauscher, S; Brustad, EM; Isaacs, FJ; Chilkoti, A
Published in: Advanced materials (Deerfield Beach, Fla.)
February 2018

Hydrogel particles are versatile materials that provide exquisite, tunable control over the sequestration and delivery of materials in pharmaceutics, tissue engineering, and photonics. The favorable properties of hydrogel particles depend largely on their size, and particles ranging from nanometers to micrometers are used in different applications. Previous studies have only successfully fabricated these particles in one specific size regime and required a variety of materials and fabrication methods. A simple yet powerful system is developed to easily tune the size of polypeptide-based, thermoresponsive hydrogel particles, from the nano- to microscale, using a single starting material. Particle size is controlled by the self-assembly and unique phase transition behavior of elastin-like polypeptides in bulk and within microfluidic-generated droplets. These particles are then stabilized through ultraviolet irradiation of a photo-crosslinkable unnatural amino acid (UAA) cotranslationally incorporated into the parent polypeptide. The thermoresponsive property of these particles provides an active mechanism for actuation and a dynamic responsive to the environment. This work represents a fundamental advance in the generation of crosslinked biomaterials, especially in the form of soft matter colloids, and is one of the first demonstrations of successful use of UAAs in generating a novel material.

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Published In

Advanced materials (Deerfield Beach, Fla.)

DOI

EISSN

1521-4095

ISSN

0935-9648

Publication Date

February 2018

Volume

30

Issue

5

Related Subject Headings

  • Phase Transition
  • Peptides
  • Nanostructures
  • Nanoscience & Nanotechnology
  • Hydrogels
  • Elastin
  • Amino Acids
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
 

Citation

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Costa, S. A., Simon, J. R., Amiram, M., Tang, L., Zauscher, S., Brustad, E. M., … Chilkoti, A. (2018). Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides. Advanced Materials (Deerfield Beach, Fla.), 30(5). https://doi.org/10.1002/adma.201704878
Costa, Simone A., Joseph R. Simon, Miriam Amiram, Lei Tang, Stefan Zauscher, Eric M. Brustad, Farren J. Isaacs, and Ashutosh Chilkoti. “Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides.Advanced Materials (Deerfield Beach, Fla.) 30, no. 5 (February 2018). https://doi.org/10.1002/adma.201704878.
Costa SA, Simon JR, Amiram M, Tang L, Zauscher S, Brustad EM, et al. Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides. Advanced materials (Deerfield Beach, Fla). 2018 Feb;30(5).
Costa, Simone A., et al. “Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides.Advanced Materials (Deerfield Beach, Fla.), vol. 30, no. 5, Feb. 2018. Epmc, doi:10.1002/adma.201704878.
Costa SA, Simon JR, Amiram M, Tang L, Zauscher S, Brustad EM, Isaacs FJ, Chilkoti A. Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides. Advanced materials (Deerfield Beach, Fla). 2018 Feb;30(5).
Journal cover image

Published In

Advanced materials (Deerfield Beach, Fla.)

DOI

EISSN

1521-4095

ISSN

0935-9648

Publication Date

February 2018

Volume

30

Issue

5

Related Subject Headings

  • Phase Transition
  • Peptides
  • Nanostructures
  • Nanoscience & Nanotechnology
  • Hydrogels
  • Elastin
  • Amino Acids
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences