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STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo.

Publication ,  Journal Article
Bazzi, H; Soroka, E; Alcorn, HL; Anderson, KV
Published in: Proc Natl Acad Sci U S A
December 19, 2017

Regulated mesoderm migration is necessary for the proper morphogenesis and organ formation during embryonic development. Cell migration and its dependence on the cytoskeleton and signaling machines have been studied extensively in cultured cells; in contrast, remarkably little is known about the mechanisms that regulate mesoderm cell migration in vivo. Here, we report the identification and characterization of a mouse mutation in striatin-interacting protein 1 (Strip1) that disrupts migration of the mesoderm after the gastrulation epithelial-to-mesenchymal transition (EMT). STRIP1 is a core component of the biochemically defined mammalian striatin-interacting phosphatases and kinase (STRIPAK) complexes that appear to act through regulation of protein phosphatase 2A (PP2A), but their functions in mammals in vivo have not been examined. Strip1-null mutants arrest development at midgestation with profound disruptions in the organization of the mesoderm and its derivatives, including a complete failure of the anterior extension of axial mesoderm. Analysis of cultured mesoderm explants and mouse embryonic fibroblasts from null mutants shows that the mesoderm migration defect is correlated with decreased cell spreading, abnormal focal adhesions, changes in the organization of the actin cytoskeleton, and decreased velocity of cell migration. The results show that STRIPAK complexes are essential for cell migration and tissue morphogenesis in vivo.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

December 19, 2017

Volume

114

Issue

51

Start / End Page

E10928 / E10936

Location

United States

Related Subject Headings

  • Phenotype
  • Mutation
  • Multiprotein Complexes
  • Morphogenesis
  • Mice
  • Mesoderm
  • Embryonic Development
  • Cell Movement
  • Carrier Proteins
  • Animals
 

Citation

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ICMJE
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Bazzi, H., Soroka, E., Alcorn, H. L., & Anderson, K. V. (2017). STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo. Proc Natl Acad Sci U S A, 114(51), E10928–E10936. https://doi.org/10.1073/pnas.1713535114
Bazzi, Hisham, Ekaterina Soroka, Heather L. Alcorn, and Kathryn V. Anderson. “STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo.Proc Natl Acad Sci U S A 114, no. 51 (December 19, 2017): E10928–36. https://doi.org/10.1073/pnas.1713535114.
Bazzi H, Soroka E, Alcorn HL, Anderson KV. STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo. Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):E10928–36.
Bazzi, Hisham, et al. “STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo.Proc Natl Acad Sci U S A, vol. 114, no. 51, Dec. 2017, pp. E10928–36. Pubmed, doi:10.1073/pnas.1713535114.
Bazzi H, Soroka E, Alcorn HL, Anderson KV. STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo. Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):E10928–E10936.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

December 19, 2017

Volume

114

Issue

51

Start / End Page

E10928 / E10936

Location

United States

Related Subject Headings

  • Phenotype
  • Mutation
  • Multiprotein Complexes
  • Morphogenesis
  • Mice
  • Mesoderm
  • Embryonic Development
  • Cell Movement
  • Carrier Proteins
  • Animals