Functional interrogation and mining of natively paired human VH:VL antibody repertoires.
Publication
, Journal Article
Wang, B; DeKosky, BJ; Timm, MR; Lee, J; Normandin, E; Misasi, J; Kong, R; McDaniel, JR; Delidakis, G; Leigh, KE; Niezold, T; Choi, CW ...
Published in: Nat Biotechnol
February 2018
We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.
Duke Scholars
Published In
Nat Biotechnol
DOI
EISSN
1546-1696
Publication Date
February 2018
Volume
36
Issue
2
Start / End Page
152 / 155
Location
United States
Related Subject Headings
- Peptide Library
- Humans
- High-Throughput Nucleotide Sequencing
- HIV-1
- HIV Infections
- HIV Antibodies
- B-Lymphocytes
- Antibodies, Neutralizing
- Antibodies, Monoclonal
- Amino Acid Sequence
Citation
APA
Chicago
ICMJE
MLA
NLM
Wang, B., DeKosky, B. J., Timm, M. R., Lee, J., Normandin, E., Misasi, J., … Georgiou, G. (2018). Functional interrogation and mining of natively paired human VH:VL antibody repertoires. Nat Biotechnol, 36(2), 152–155. https://doi.org/10.1038/nbt.4052
Wang, Bo, Brandon J. DeKosky, Morgan R. Timm, Jiwon Lee, Erica Normandin, John Misasi, Rui Kong, et al. “Functional interrogation and mining of natively paired human VH:VL antibody repertoires.” Nat Biotechnol 36, no. 2 (February 2018): 152–55. https://doi.org/10.1038/nbt.4052.
Wang B, DeKosky BJ, Timm MR, Lee J, Normandin E, Misasi J, et al. Functional interrogation and mining of natively paired human VH:VL antibody repertoires. Nat Biotechnol. 2018 Feb;36(2):152–5.
Wang, Bo, et al. “Functional interrogation and mining of natively paired human VH:VL antibody repertoires.” Nat Biotechnol, vol. 36, no. 2, Feb. 2018, pp. 152–55. Pubmed, doi:10.1038/nbt.4052.
Wang B, DeKosky BJ, Timm MR, Lee J, Normandin E, Misasi J, Kong R, McDaniel JR, Delidakis G, Leigh KE, Niezold T, Choi CW, Viox EG, Fahad A, Cagigi A, Ploquin A, Leung K, Yang ES, Kong W-P, Voss WN, Schmidt AG, Moody MA, Ambrozak DR, Henry AR, Laboune F, Ledgerwood JE, Graham BS, Connors M, Douek DC, Sullivan NJ, Ellington AD, Mascola JR, Georgiou G. Functional interrogation and mining of natively paired human VH:VL antibody repertoires. Nat Biotechnol. 2018 Feb;36(2):152–155.
Published In
Nat Biotechnol
DOI
EISSN
1546-1696
Publication Date
February 2018
Volume
36
Issue
2
Start / End Page
152 / 155
Location
United States
Related Subject Headings
- Peptide Library
- Humans
- High-Throughput Nucleotide Sequencing
- HIV-1
- HIV Infections
- HIV Antibodies
- B-Lymphocytes
- Antibodies, Neutralizing
- Antibodies, Monoclonal
- Amino Acid Sequence