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Disruption of thyroid hormone sulfotransferase activity by brominated flame retardant chemicals in the human choriocarcinoma placenta cell line, BeWo.

Publication ,  Journal Article
Leonetti, CP; Butt, CM; Stapleton, HM
Published in: Chemosphere
April 2018

Brominated flame retardants (BFRs) have been shown to disrupt thyroid hormone (TH) homeostasis through multiple mechanisms, including inhibition of enzymes that regulate intracellular levels of THs, such as sulfotransferases (SULTs). The placenta plays a critical role in helping to maintain TH levels during fetal development and expresses SULTs. This is concerning given that disruption of TH regulation within the placenta could potentially harm the developing fetus. In this study, we investigated the effects of two polybrominated diphenyl ethers (PBDEs), two hydroxylated PBDEs, and 2,4,6-tribromophenol (2,4,6-TBP) on TH SULT activity in a choriocarcinoma placenta cell line (BeWo). BeWo cells were exposed to BFR concentrations up to 1 μM for 1-24 h to investigate changes in basal SULT activity and in mRNA expression of several TH regulating genes. 2,4,6-TBP was the most potent inhibitor of basal 3,3'-T2 SULT activity at all exposure durations, decreasing activity by as much as 86% after 24 h of exposure. BDE-99, 3-OH BDE-47, and 6-OH BDE-47 also decreased 3,3'-T2 SULT activity by 23-42% at concentrations of 0.5 μM and 1.0 μM following 24 h exposures. BDE-47 had no effect on SULT activity, and there was no observed effect of any BFR exposure on expression of SULT1A1, or thyroid nuclear receptors alpha or beta. This research demonstrates that total TH SULT activity in placental cells are sensitive to BFR exposure; however, the mechanisms and consequences have yet to be fully elucidated.

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Published In

Chemosphere

DOI

EISSN

1879-1298

ISSN

0045-6535

Publication Date

April 2018

Volume

197

Start / End Page

81 / 88

Related Subject Headings

  • Thyroid Hormones
  • Thyroid Gland
  • Sulfotransferases
  • Protein Binding
  • Pregnancy
  • Polybrominated Biphenyls
  • Placenta
  • Phenols
  • Meteorology & Atmospheric Sciences
  • Hydroxylation
 

Citation

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Leonetti, C. P., Butt, C. M., & Stapleton, H. M. (2018). Disruption of thyroid hormone sulfotransferase activity by brominated flame retardant chemicals in the human choriocarcinoma placenta cell line, BeWo. Chemosphere, 197, 81–88. https://doi.org/10.1016/j.chemosphere.2017.12.172
Leonetti, Christopher P., Craig M. Butt, and Heather M. Stapleton. “Disruption of thyroid hormone sulfotransferase activity by brominated flame retardant chemicals in the human choriocarcinoma placenta cell line, BeWo.Chemosphere 197 (April 2018): 81–88. https://doi.org/10.1016/j.chemosphere.2017.12.172.
Leonetti, Christopher P., et al. “Disruption of thyroid hormone sulfotransferase activity by brominated flame retardant chemicals in the human choriocarcinoma placenta cell line, BeWo.Chemosphere, vol. 197, Apr. 2018, pp. 81–88. Epmc, doi:10.1016/j.chemosphere.2017.12.172.
Journal cover image

Published In

Chemosphere

DOI

EISSN

1879-1298

ISSN

0045-6535

Publication Date

April 2018

Volume

197

Start / End Page

81 / 88

Related Subject Headings

  • Thyroid Hormones
  • Thyroid Gland
  • Sulfotransferases
  • Protein Binding
  • Pregnancy
  • Polybrominated Biphenyls
  • Placenta
  • Phenols
  • Meteorology & Atmospheric Sciences
  • Hydroxylation